ab10760 staining CCN1 in mouse kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded). Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in Tris-EDTA buffer pH9.0 and bloking for 15 minutes at 20°C (5 minutes/peroxidase block and 10 minutes/protein block). The primary antibody was diluted 1/100 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used, undiluted as the secondary. See Abreview
All lanes : Anti-CCN1 antibody (ab10760) at 1 µg/mlLane 1 : Liver (Mouse) Tissue LysateLane 2 : Heart (Mouse) Tissue LysateLane 3 : Kidney (Mouse) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
All lanes : Anti-CCN1 antibody (ab10760) at 1/500 dilutionLane 1 : Hela nuclear cell extractLane 2 : Hela whole cell lysateLane 3 : A431 whole cell lysateLane 4 : Jurkat whole cell lysateLysates/proteins at 20 µg per lane.SecondaryAlexa Fluor Goat polyclonal to Rabbit IgG at 1/5000 dilution