![Anti-CCN1 antibody [3H3] (ab80112) at 1/250 dilution + Jurkat cell lysate at 40 µgSecondarygoat anti-mouse HRPdeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/22684_CCN1-Primary-antibodies-ab80112-1.jpg)
Anti-CCN1 antibody [3H3] (ab80112) at 1/250 dilution + Jurkat cell lysate at 40 µgSecondarygoat anti-mouse HRPdeveloped using the ECL technique
ab80112 at 1/50 dilution staining CCN1 in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) staining technique.
ICC/IF image of ab80112 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80112, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.