Anti-CCR4 antibody
| Name | Anti-CCR4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab1669 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | FC ICC/IF ICC/IF ELISA ICC/IF IHC-P WB IHC-F |
| Species Reactivities | Mouse, Human, Monkey |
| Antigen | Synthetic peptide: SNYYLYESIPKPCTKEGIKAFGE , corresponding to amino acids 17-39 of Human CCR4 (extracellular domain) |
| Description | Goat Polyclonal |
| Gene | CCR4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry using ab1669 on a section of human spleen.
Anti-CCR4 antibody (ab1669) at 1 µg/ml + Thymus (Human) Tissue Lysate - adult normal tissue (ab30146) at 10 µgSecondaryRabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of CCR4 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1669, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab1669 staining CCR4 in Mouse whole blood by Flow Cytometry. Red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide and fixed in paraformaldehyde. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C. A biotin-conjugated Donkey anti-goat IgG polyclonal (1/200) was used as the secondary antibody. Gating Strategy: Live Lymphocytes.See Abreview
Product References
Identification of chemoattractive factors involved in the migration of bone - Identification of chemoattractive factors involved in the migration of bone
Song CH, Honmou O, Furuoka H, Horiuchi M. J Virol. 2011 Nov;85(21):11069-78.
The chemokine receptor CCR4 promotes tumor growth and lung metastasis in breast - The chemokine receptor CCR4 promotes tumor growth and lung metastasis in breast
Li JY, Ou ZL, Yu SJ, Gu XL, Yang C, Chen AX, Di GH, Shen ZZ, Shao ZM. Breast Cancer Res Treat. 2012 Feb;131(3):837-48.
Chemokine and cytokine mediated loss of regulatory T cells in lymph nodes during - Chemokine and cytokine mediated loss of regulatory T cells in lymph nodes during
Qin S, Sui Y, Soloff AC, Junecko BA, Kirschner DE, Murphey-Corb MA, Watkins SC, Tarwater PM, Pease JE, Barratt-Boyes SM, Reinhart TA. J Immunol. 2008 Apr 15;180(8):5530-6.
Chemokine CXCL10 promotes atherogenesis by modulating the local balance of - Chemokine CXCL10 promotes atherogenesis by modulating the local balance of
Heller EA, Liu E, Tager AM, Yuan Q, Lin AY, Ahluwalia N, Jones K, Koehn SL, Lok VM, Aikawa E, Moore KJ, Luster AD, Gerszten RE. Circulation. 2006 May 16;113(19):2301-12. Epub 2006 May 8.
Chemokine receptor expression in cutaneous T cell and NK/T-cell lymphomas: - Chemokine receptor expression in cutaneous T cell and NK/T-cell lymphomas:
Yagi H, Seo N, Ohshima A, Itoh T, Itoh N, Horibe T, Yoshinari Y, Takigawa M, Hashizume H. Am J Surg Pathol. 2006 Sep;30(9):1111-9.
Elevated expression of TARC (CCL17) and MDC (CCL22) in models of cigarette - Elevated expression of TARC (CCL17) and MDC (CCL22) in models of cigarette
Ritter M, Goggel R, Chaudhary N, Wiedenmann A, Jung B, Weith A, Seither P. Biochem Biophys Res Commun. 2005 Aug 19;334(1):254-62.