Spectral Confocal Microscopy of CHO cells using ab59550 at 1 µg/106 cells . CHO cells were transiently transfected with an expression vector encoding CCR4. Binding of ab59550 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
FACS analysis of BOSC23 cells using ab59550 at 1.2 µg/ 106 cells. BOSC23 cells were transiently transfected with an expression vector encodingeither CCR4 (red curve) or an irrelevant protein(control transfectant). Binding of ab59550 was detected with a PE conjugated secondary antibody.
SDS-PAGE analysis of purified ab59550. Lane 1: molecular weight marker, Lane 2: 2 µg of purified antibody.