Anti-CCR7 antibody [Y59]
| Name | Anti-CCR7 antibody [Y59] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32527 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | Y59 |
| Applications | WB IHC-P ICC/IF ICC/IF IP |
| Species Reactivities | Mouse, Rat, Human, Monkey |
| Antigen | A synthetic peptide corresponding to residues in N-terminal extracellular domain of human CKR7 |
| Description | Rabbit Monoclonal |
| Gene | CCR7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-CCR7 antibody [Y59] (ab32527) at 1/5000 dilutionLane 1 : K562 cell lysateLane 2 : Daudi cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/22871_ab32527wb.jpg)
All lanes : Anti-CCR7 antibody [Y59] (ab32527) at 1/5000 dilutionLane 1 : K562 cell lysateLane 2 : Daudi cell lysate
Immunohistochemical analysis of paraffin-embedded human spleen using ab32527 at 1/250 dilution.
Immunofluorescent staining of HeLa cells using ab32527 at 1/250 dilution.
ICC/IF image of ab32527 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32527, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Effect of CCR7, CXCR4 and VEGF-C on the lymph node metastasis of human pancreatic - Effect of CCR7, CXCR4 and VEGF-C on the lymph node metastasis of human pancreatic
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Macrophage polarization following chitosan implantation. - Macrophage polarization following chitosan implantation.
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Role of CCL19/21 and its possible signaling through CXCR3 in development of - Role of CCL19/21 and its possible signaling through CXCR3 in development of
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HDL promotes rapid atherosclerosis regression in mice and alters inflammatory - HDL promotes rapid atherosclerosis regression in mice and alters inflammatory
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Polysialylated neuropilin-2 enhances human dendritic cell migration through the - Polysialylated neuropilin-2 enhances human dendritic cell migration through the
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Expression of master regulators of helper T-cell differentiation in peripheral - Expression of master regulators of helper T-cell differentiation in peripheral
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IRAK-M removal counteracts dendritic cell vaccine deficits in migration and - IRAK-M removal counteracts dendritic cell vaccine deficits in migration and
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Constitutive expression of the alpha4 integrin correlates with tumorigenicity and - Constitutive expression of the alpha4 integrin correlates with tumorigenicity and
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Acute in vivo exposure to interferon-gamma enables resident brain dendritic cells - Acute in vivo exposure to interferon-gamma enables resident brain dendritic cells
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