ab82073, at 1/100 dilution, staining of Formalin-fixed, paraffin-embedded Human small intestine by Immunohistochemistry
ab82073 staining CD10 in human MSC cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized. The sample was incubated with the primary antibody (1/500) for 1 hour at 20°C. A PerCP-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.Gating Strategy: isotype population (shown in white).See Abreview
Anti-CD10 antibody (ab82073) at 1 µg/ml + Kidney (Human) Tissue Lysate - adult normal tissue (ab30203) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.