Anti-CD146 antibody [EPR3208]

• Supplier: Abcam
• Catalog: ab75769
• Prices: $401.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3208
• Applications: ICC/IF ICC/IF IHC-F WB IP IHC-P ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (Human), intracellular sequence
• Description: Rabbit Monoclonal
• Gene: MCAM
• Conjugate: Unconjugated

Product images

ab75769 at 1/250 dilution staining CD146 in melanoma tissue, using a HRP/AP polymerized secondary antibody.

ab75769 staining CD146 in mouse spleen tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with acetone and blocked with 5% serum for 2 hours at 25°C. The sample was incubated with primary antibody (1/250 in PBS-Tween) at 4°C for 12 hours. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG (1/250) was used as secondary antibody. Nuclear staining was with DAPI.See Abreview

ab75769 staining CD146 in murine bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in methanol and then blocked using 5% serum for 2 hours at 25°C. Samples were then incubated with the primary antibody at 1/250 for 12 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution. Counterstained with DAPI.See Abreview

ab75769 showing positive staining in Breast carcinoma vessels tissue.

ab75769 showing positive staining in Urinary bladder transitional carcinoma vessels tissue.

ab75769 showing positive staining in Glioma vessels tissue.

ab75769 showing positive staining in Normal tonsil tissue.

ab75769 showing positive staining in Normal spleen tissue.

Product References

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