Anti-CD146 antibody [P1H12]
| Name | Anti-CD146 antibody [P1H12] |
|---|---|
| Supplier | Abcam |
| Catalog | ab24577 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | P1H12 |
| Applications | ICC/IF ICC/IF IHC-P WB ELISA IHC-F IP FC |
| Species Reactivities | Human, Mouse, Dog |
| Antigen | Tissue/ cell preparation: human umbilical vein endothelial cells (HUVECs) |
| Description | Mouse Monoclonal |
| Gene | MCAM |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of CD146 staining in human aorta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24577, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![All lanes : Anti-CD146 antibody [P1H12] (ab24577) at 5 µg/mlLane 1 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate Lane 2 : Blood Vessel: artery (Human) Membrane Lysate - adult normal tissue (ab28989)Lysates/proteins at 25 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutionPerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/23373_CD146-Primary-antibodies-ab24577-14.jpg)
All lanes : Anti-CD146 antibody [P1H12] (ab24577) at 5 µg/mlLane 1 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate Lane 2 : Blood Vessel: artery (Human) Membrane Lysate - adult normal tissue (ab28989)Lysates/proteins at 25 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutionPerformed under reducing conditions.
ab24577 at 1/1000 dilution staining mouse brain tissue sections by Immunohistochemistry (Frozen sections). Mice were processed by transcardial perfusion first with saline, then 4% PF. After overnight incubation in 4% PF, brains were transfered to sucrose. Upon saturation, brains were frozen, sectioned with a cryostat, and then the sections were immediately mounted on slides. The tissue was incubated with ab24577 for 2 hours and then an Alexa Fluor ® 594 goat anti-mouse IgG was used as the secondary (red). DAPI staining is shown in blue. Images were taken with a confocal microscope in comparable cortex regions of the lesion or contralateral side in the same section. The lesion image shown is from this model of ischemia-hypoxia, with 1 hour of recovery time after injury, when endothelial cell activation is quite robust.See Abreview
ab24577 staining CD146 in murine bone marrow leukocytes by Immunocytochemistry/ Immunofluorescence. The cells were fixed in methanol and then blocked using 5% serum for 2 hours at 25°C. Samples were then incubated with the primary antibody at 1/400 for 12 hours at 4°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 594 (red) used at a 1/500 dilution.See Abreview
Flow cytometry analysis of hBMSCs cultured in complete a-MEM supplemented with 1 ng/mL TGF-ß1 for 7 days, staining CD146 with ab24577. Cells were trypsinized, counted and resuspended in 2% BSA at a concentration of 2500 cells/µL. Cells were first incubated for 45 min at 4°C, protected from light, with primary antibody antibody (1/100). Cells were then incubated for 45 minutes, protected from light, at room temperature with AlexaFluor®488-conjugated goat anti-mouse secondary antibody (1/500).
Product References
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