Anti-CD163 antibody

• Supplier: Abcam
• Catalog: ab87099
• Prices: $385.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P WB ICC/IF ICC/IF
• Species Reactivities: Human, Pig, Chimpanzee, Monkey, Ape, Orangutan
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 1050 - 1150 of Human CD163
• Description: Rabbit Polyclonal
• Gene: CD163
• Conjugate: Unconjugated

Product images

IHC image of CD163 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87099, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

ICC/IF image of ab87099 stained Jeg3 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87099, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

All lanes : Anti-CD163 antibody (ab87099) at 2 µg/ml (3% milk)Lane 1 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 2 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

All lanes : Anti-CD163 antibody (ab87099) at 1 µg/mlLane 1 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 2 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Product References

Mutation of NLRC4 causes a syndrome of enterocolitis and autoinflammation. - Mutation of NLRC4 causes a syndrome of enterocolitis and autoinflammation.

Romberg N, Al Moussawi K, Nelson-Williams C, Stiegler AL, Loring E, Choi M, Overton J, Meffre E, Khokha MK, Huttner AJ, West B, Podoltsev NA, Boggon TJ, Kazmierczak BI, Lifton RP. Nat Genet. 2014 Oct;46(10):1135-9.

Adenosine A2A receptor activation prevents wear particle-induced osteolysis. - Adenosine A2A receptor activation prevents wear particle-induced osteolysis.

Mediero A, Frenkel SR, Wilder T, He W, Mazumder A, Cronstein BN. Sci Transl Med. 2012 May 23;4(135):135ra65.