CD163

Gene Symbol CD163
Entrez Gene 9332
Alt Symbol M130, MM130
Species Human
Gene Type protein-coding
Description CD163 molecule
Other Description hemoglobin scavenger receptor|macrophage-associated antigen|scavenger receptor cysteine-rich type 1 protein M130
Swissprots Q07900 Q07898 Q07899 Q07901 Q86VB7 Q2VLH7 C9JIG2
Accessions CAB45233 EAW88662 EAW88663 EAW88664 EAW88665 EAW88666 Q86VB7 AA962842 AI128043 AI129762 AK226017 BC051281 AAH51281 DA619387 DB291765 DQ058615 AAY99762 Z22968 CAA80541 Z22969 CAA80542 Z22970 CAA80543 Z22971 CAA80544 XM_005253528 XP_005253585 XM_005253529 XP_005253586 NM_004244 NP_004235 NM_203416 NP_981961
Function Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
Subcellular Location Soluble CD163: Secreted.
Tissue Specificity Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood. {ECO:0000269|PubMed:10577520, ECO:0000269|PubMed:11196644, ECO:0000269|PubMed:16434690}.

CD163 (H-130) - sc-33559 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CD163 (H-130): sc-33559. Western blot analysis of CD163 expression in THP-1 (A) and CCRF-CEM (B) whole cell lysates.

CD163 (GHI/61) - sc-20066 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD 163 (GHI/61) PE: sc-20066 PE. FCM analysis of human peripheral blood leukocytes. Black line histogram represents the isotype control: normal mouse IgG<sub>1</sub>: 2866.

CD163 (C-16) - sc-18794 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CD163 (C-16): sc-18794. Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in red pulp.

CD163 (K-18) - sc-18796 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

CD163 (RM3/1) - sc-33715 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD163 (RM3/1): sc-33715. Indirect FCM analysis of human peripheral blood leukocytes stained with CD163 (RM3/1), followed by PE-conjugated goat anti-mouse IgG<sub>1</sub>: sc-3764. Black line histogram represents the isotype control, normal mouse IgG<sub>1</sub>: sc-3877.

Anti-CD163 antibody - ab87099 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of CD163 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87099, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CD163 antibody - ab183476 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of CD163 staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab183476, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CD163 antibody - ab174867 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab174867 stained Jeg3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab174867 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CD163 antibody - ab79610 from Abcam

Host Chicken
Clonality Polyclonal
ab79610, at 4 µg/ml, staining CD163 in frozen human placenta tissue sections by Immunohistochemistry.

Anti-CD163 antibody - ab85182 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-CD163 antibody (ab85182) at 1/50 dilution + K-562 cell line lysate at 35 µg

Anti-CD163 antibody [10D6] - ab201461 from Abcam

Prices $382.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 10D6

Anti-CD163 antibody [10D6], prediluted - ab74604 from Abcam

Prices $382.00
Sizes 7 ml
Host Mouse
Clonality Monoclonal
Clone 10D6
ab74604 staining CD163 in human placenta by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and AEC chromogen. Note membrane staining of monocytes and macrophages

Anti-CD163 antibody [2C7] - ab194944 from Abcam

Prices $384.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 2C7
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, labeling CD163 using ab194944 at 10 µg/mL.

Anti-CD163 antibody [2G12] - ab156769 from Abcam

Prices $394.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2G12
All lanes : Anti-CD163 antibody [2G12] (ab156769) at 1/1000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CD163 cDNALysates/proteins at 5 µg per lane.

Anti-CD163 antibody [3B4] - ab192666 from Abcam

Prices $384.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 3B4
All lanes : Anti-CD163 antibody [3B4] (ab192666) at 1/200 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with pCMV6-ENTRY CD163 cDNALysates/proteins at 5 µg per lane.

Anti-CD163 antibody [EPR14336] - ab199427 from Abcam

Prices $347.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14336
All lanes : Anti-CD163 antibody [EPR14336] (ab199427) at 1/2000 dilutionLane 1 : Human spleen lysateLane 2 : Human fetal liver lysateLane 3 : Human placenta lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Anti-CD163 antibody [EPR14643] - C-terminal - ab188571 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14643
All lanes : Anti-Gastric Inhibitory Polypeptide Receptor antibody - N-terminal (ab188591) at 1/1000 dilutionLane 1 : Human fetal liver lysateLane 2 : Human placenta lysateLane 3 : Human breast cancer lysateLane 4 : Human spleen lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution

Anti-CD163 antibody [EPR14643-36] - C-terminal - ab189915 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14643-36
Immunoprecipitation. ab189915 at 1/1000 labeling CD163 in Human fetal kidney lysate immunoprecipitated using ab189915 at 1/50.

Anti-CD163 antibody [GHI/61] - ab111250 from Abcam

Prices $385.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone GHI/61
Formalin-Fixed, Paraffin-Embedded Human Placenta tissue stained using ab111250 at a concentration of 20 µg/ml.

Anti-CD163 antibody [GHI/61] (Biotin) - ab134417 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone GHI/61
Flow cytometric analysis of CD163 in Human peripheral blood monocytes stained with ab134417 at 0.5 µg per 106 cells (in 100 µl volume), followed by Sav-PE.

Anti-CD163 antibody [GHI/61] (Phycoerythrin) - ab95613 from Abcam

Prices $189.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone GHI/61
Staining of normal Human peripheral blood cells with Mouse IgG1 ? Isotype Control PE (open histogram) or ab95613 (filled histogram). Cells in the monocyte gate were used for analysis.

Anti-CD163 antibody [GHI/61], prediluted (Allophycocyanin) - ab134416 from Abcam

Prices $186.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone GHI/61

Anti-CD163 antibody [MM0161-6J58] - ab89548 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone MM0161-6J58

Anti-CD163 antibody [RM3/1] - ab17051 from Abcam

Prices $379.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone RM3/1

Anti-CD163 antibody [RM3/1] (Biotin) - ab15676 from Abcam

Prices $189.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone RM3/1
Overlay histogram showing JEG3 cells stained with ab15676 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15676, 2µg/1x106 cells) for 30 min at 22ºC followed by Streptavidin-FITC (1µg/1x106 cells). Isotype control antibody (black line) was mouse IgG1 (FITC) (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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