Anti-CD163 antibody
| Name | Anti-CD163 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab183476 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Human, Pig, Chimpanzee, Monkey, Ape, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1050 - 1150 of Human CD163 |
| Description | Rabbit Polyclonal |
| Gene | CD163 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of CD163 staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab183476, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-CD163 antibody (ab183476) at 1 µg/ml + Spleen (Human) Tissue Lysate - adult normal tissue (ab29699) at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.