Anti-CD18 (phospho S745) antibody [EP1288Y]

• Supplier: Abcam
• Catalog: ab52920
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1288Y
• Applications: WB IP IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic phospho-peptide corresponding to residues surrounding Ser745 of human CD18
• Description: Rabbit Monoclonal
• Gene: ITGB2
• Conjugate: Unconjugated

Product images

ICC/IF image of ab52920 stained Raw 246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab52920 at 1/1000 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

All lanes : Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 1/500 dilutionLane 1 : Jurkat cell lysate, untreatedLane 2 : Jurkat cell lysate, treated with AP.Lysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution

Ab52920 (1:250) staining human CD18 in human tonsil tissue by immunohistochemistry using paraffin embedded tissue.Ab52920 (1:250) staining human CD18 in human tonsil tissue by immunohistochemistry using paraffin embedded tissue.

Human peripheral blood lymphocytes stained with ab52920 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab52920, 1/1000 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blo