![All lanes : Anti-CD1a antibody [7A7] (ab113618) at 1/500 dilutionLane 1 : K562 cell lysateLane 2 : Raji cell lysateLane 3 : MOLT 4 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/23738_CD1a-Primary-antibodies-ab113618-1.jpg)
All lanes : Anti-CD1a antibody [7A7] (ab113618) at 1/500 dilutionLane 1 : K562 cell lysateLane 2 : Raji cell lysateLane 3 : MOLT 4 cell lysate
ab113618 at 1/200 dilution staining CD1a in Paraffin-embedded Human cervical cancer tissue by Immunohistochemistry followed by DAB staining.
ab113618 at 1/200 dilution staining CD1a in Paraffin-embedded Human colon cancer tissue by Immunohistochemistry followed by DAB staining.
ab113618 at 1/200 dilution staining CD1a in Paraffin-embedded Human brain tissue by Immunohistochemistry followed by DAB staining.
ab113618 at 1/200 dilution staining CD1a in Paraffin-embedded Human submaxillary tumor tissue by Immunohistochemistry followed by DAB staining.
![Overlay histogram showing MOLT4 cells stained with ab113618 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab113618, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed MOLT4 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/23743_CD1a-Primary-antibodies-ab113618-9.jpg)
Overlay histogram showing MOLT4 cells stained with ab113618 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab113618, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed MOLT4 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
ab113618 at 1/200 dilution staining CD1a in Raji cells by Immunofluorescence (green). DRAQ5 fluorescent DNA dye (blue).