Anti-CD22 antibody [2H1C4] (ab181771) at 1/500 dilution + Human CD22 recombinant protein
All lanes : Anti-CD22 antibody [2H1C4] (ab181771) at 1/500 dilutionLane 1 : HEK293 cell lysate, non-transfectedLane 2 : HEK293 cell lysate, transfected with CD22 (amino acids 622-725)-hIgGFc
All lanes : Anti-CD22 antibody [2H1C4] (ab181771) at 1/500 dilutionLane 1 : mouse L1210 cell lysateLane 2 : HeLa cell lysateLane 3 : HEK293 cell lysateLane 4 : Jurkat cell lysateLane 5 : OCM-1 cell lysateLane 6 : A432 cell lysateLane 7 : mouse NIH 3T3 cell lysate
Immunofluorescent analysis of HeLa cells, labeling CD22 with ab181771 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of HeLa cells labeling CD22 with ab181771 at 1/200 dilution (green); negative control (red).
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling CD22 with ab181771 at 1/200 dilution followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling CD22 with ab181771 at 1/200 dilution followed by DAB staining.