All lanes : Anti-CD26 antibody (ab86806) at 1 µg/mlLane 1 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)Lane 2 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)Lane 3 : Breast (Human) Tissue Lysate - normal tissue (ab30090)Lane 4 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-CD26 antibody (ab86806) at 1 µg/mlLane 1 : Active human CD26 full length protein (ab79138) at 0.1 µgLane 2 : Active human CD26 full length protein (ab79138) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 10 seconds