Anti-CD2AP antibody

• Supplier: Abcam
• Catalog: ab32741
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human CD2AP
• Description: Rabbit Polyclonal
• Gene: CD2AP
• Conjugate: Unconjugated

Product images

All lanes : Anti-CD2AP antibody (ab32741) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lysates/proteins at 20 µg per lane.SecondaryIR Dye 680 Conjugated Goat Anti-Rabbit IgG (H&L) at 1/15000 dilutionPerformed under reducing conditions.

All lanes : Anti-CD2AP antibody (ab32741) at 1 µg/mlLane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 2 : Spinal Cord (Mouse) Tissue Lysate Lane 3 : Brain (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

ICC/IF image of ab32741 stained mouse embryonic fibroblast cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab32741, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

IHC image of CD2AP staining in Human normal liver formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32741, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Product References

Albuminuria and glomerular damage in mice lacking the metabotropic glutamate - Albuminuria and glomerular damage in mice lacking the metabotropic glutamate

Puliti A, Rossi PI, Caridi G, Corbelli A, Ikehata M, Armelloni S, Li M, Zennaro C, Conti V, Vaccari CM, Cassanello M, Calevo MG, Emionite L, Ravazzolo R, Rastaldi MP. Am J Pathol. 2011 Mar;178(3):1257-69.

Low-level C-reactive protein levels exert cytoprotective actions on human - Low-level C-reactive protein levels exert cytoprotective actions on human

Pawluczyk IZ, Yang B, Patel SR, Saleem MA, Topham PS. Nephrol Dial Transplant. 2011 Aug;26(8):2465-75.