Western blot analysis of extracts from HeLa cells, serum-starved overnight and either untreated or H 2 O 2 -treated (4 mM, 30 min), using Phospho-AP2M1 (Thr156) (D4F3) Rabbit mAb (upper) or β-Actin Antibody #4967 (lower).
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or H 2 O 2 -treated (4 mM, 15 min; right), using Phospho-AP2M1 (Thr156) (D4F3) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).