AP2M1

Gene Symbol AP2M1
Entrez Gene 1173
Alt Symbol AP50, CLAPM1, mu2
Species Human
Gene Type protein-coding
Description adaptor-related protein complex 2, mu 1 subunit
Other Description AP-2 complex subunit mu|AP-2 mu 2 chain|AP-2 mu chain|HA2 50 kDA subunit|adapter-related protein complex 2 mu subunit|adapter-related protein complex 2 subunit mu|adaptin-mu2|adaptor protein complex AP-2 subunit mu|adaptor-related protein complex 2 subunit mu|clathrin adaptor complex AP2, mu subunit|clathrin assembly protein complex 2 medium chain|clathrin assembly protein complex 2 mu medium chain|clathrin coat adaptor protein AP50|clathrin coat assembly protein AP50|clathrin coat-associated protein AP50|clathrin-associated/assembly/adaptor protein, medium 1|plasma membrane adaptor AP-2 50 kDa protein|plasma membrane adaptor AP-2 50kDA protein
Swissprots D3DNT1 A6NE12 Q96CW1
Accessions CAR81017 CBX84495 EAW78290 EAW78291 EAW78292 Q96CW1 AK057883 AK294159 BAG57482 AK295999 BAG58773 AK297578 BAH12618 AK297849 BAG60181 AK300228 BAG61996 AK304510 BAG65316 AK310409 BC004996 AAH04996 BC013796 AAH13796 BC014030 AAH14030 BI461415 BT007308 AAP35972 D63475 BAA09762 DQ892876 ABM83802 DQ895915 ABM86841 U36188 AAA93254 NM_001025205 NP_001020376 NM_004068 NP_004059
Function Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to p
Subcellular Location Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV. {ECO:0000250}.
Top Pathways Huntington's disease, Endocytosis, Endocrine and other factor-regulated calcium reabsorption, Synaptic vesicle cycle

Phospho-AP2M1 (Thr156) (D4F3) Rabbit mAb - 7399 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4F3
Western blot analysis of extracts from HeLa cells, serum-starved overnight and either untreated or H 2 O 2 -treated (4 mM, 30 min), using Phospho-AP2M1 (Thr156) (D4F3) Rabbit mAb (upper) or β-Actin Antibody #4967 (lower).

Phospho-AP2M1 (Thr156) Antibody - 3843 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, serum-starved overnight and either untreated or H 2 O 2 -treated (4 mM, 30 minutes), using Phospho-AP2M1 (Thr156) Antibody (upper) or an AP2M1 rabbit mAb (lower).

AP-2µ1 (H-112) - sc-99026 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

AP-2µ1 (K-13) - sc-49150 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AP-2μ1 (K-13): sc-49150. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing membrane and cytoplasmic staining of Purkinje cells, and cytoplasmic staining of cells in granular layer and cells in molecular layer.

AP-2µ1 (N-16) - sc-49151 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

AP-2µ1 (Q-20) - sc-49152 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-AP2M1 (phospho T156) antibody [EPR4700] - ab109397 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4700
All lanes : Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) at 1/1000 dilutionLane 1 : 293T cell lysate Lane 2 : 293T cell lysate treated with Calyculin ALysates/proteins at 10 µg per lane.

Anti-AP2M1 antibody - ab106542 from Abcam

Prices $385.00
Sizes 50 µg
Host Chicken
Clonality Polyclonal
Lane 1 : Anti-AP2M1 antibody (ab106542) at 1 µg/mlLane 2 : Anti-AP2M1 antibody (ab106542) at 2 µg/mlLane 1 : Human kidney tissue lysateLane 2 : Human kidney tissue lysateLysates/proteins at 15 µg per lane.

Anti-AP2M1 antibody - ab96679 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AP2M1 antibody (ab96679) at 1/500 dilutionLane 1 : 293T whole cell lysateLane 2 : HeLa S3 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-AP2M1 antibody - ab124441 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AP2M1 antibody (ab124441) at 1/1000 dilutionLane 1 : MCF-7 cell lysateLane 2 : K562 cell lysate

Anti-AP2M1 antibody [1C12] - ab119027 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1C12
All lanes : Anti-AP2M1 antibody [1C12] (ab119027) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY AP2M1 cDNALysates/proteins at 5 µg per lane.

Anti-AP2M1 antibody [EP2695Y] - ab75995 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2695Y
All lanes : Anti-AP2M1 antibody [EP2695Y] (ab75995) at 1/1000 dilutionLane 1 : MCF-7 cell lysateLane 2 : SKBR-3 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti rabbit at 1/2000 dilution

Anti-AP50 / AP2M1 Antibody - LS-C332115 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded rat kidney using AP2M1 antibody at dilution of 1:100 (200x lens).

Anti-AP50 / AP2M1 Antibody (aa97-383, clone 2D10) - LS-C337389 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2D10
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AP2M1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AP2M1.

Anti-AP50 / AP2M1 Antibody (aa97-383, clone 1C8) - LS-C337386 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1C8
Anti-AP2M1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AP2M1.

Anti-AP50 / AP2M1 Antibody (aa97-383, clone 1B11) - LS-C337387 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B11
Immunofluorescent staining of HeLa cells using anti-AP2M1 mouse monoclonal antibody.

Anti-AP50 / AP2M1 Antibody (aa97-383, clone 1E9) - LS-C337385 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1E9
IHC of paraffin-embedded Human colon tissue using anti-AP2M1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Anti-AP50 / AP2M1 Antibody (phospho-Thr156, clone EPR4700) - LS-C138107 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4700
Western blot analysis on 293T cell lysates using anti-Phospho-AP2 mu1 (pT156) antibody. Cells were either (A) untreated (B) treated with Calyculin A.

Anti-AP50 / AP2M1 Antibody - LS-C109514 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Sample (30 ug of whole cell lysate). A: Hep G2. 10% SDS PAGE. AP2M1 antibody diluted at 1:1000.

Anti-AP50 / AP2M1 Antibody (FITC) - LS-C183089 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-AP50 / AP2M1 Antibody (Biotin) - LS-C183090 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-AP50 / AP2M1 Antibody (C-Terminus) - LS-C108514 from LifeSpan Bioscience

Prices $345.00, $1,035.00
Sizes 100 µg, 500 µg
Host Chicken
Clonality Polyclonal
Immunofluorescence of AP2M1 in Mouse Kidney cells with AP2M1 antibody at 20 ug/ml.

Anti-AP50 / AP2M1 Antibody (aa11-215) - LS-C178947 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-AP50 / AP2M1 Antibody (aa387-415) - LS-C156389 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
AP2M1 Antibody western blot of mouse Neuro-2a cell line lysates (35 ug/lane). The AP2M1 antibody detected the AP2M1 protein (arrow).

Anti-AP50 / AP2M1 Antibody (clone 1C12) - LS-C172774 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1C12
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AP2M1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AP2M1.
Search more