All lanes : Anti-CD36 antibody (ab64014) at 1 µg/mlLane 1 : Human Platelet (Human adult normal cell line) Whole Cell Lysate Lane 2 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 3 : Adipose (Human) Total Protein Lysate - adult normal tissue (ab28980)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-CD36 antibody (ab64014) at 2 µg/ml (Blocked in 3% milk)Lane 1 : Heart (Mouse) Tissue Lysate Lane 2 : Heart (Rat) Tissue Lysate Lane 3 : Adult Mouse Brown Adipose Tissue Tissue Lysate Lane 4 : Adult Rat Brown Adipose Tissue Tissue Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution