Anti-CD36 antibody [EPR6573] (ab133625) at 1/100 dilution (unpurified) + Rat heart tissue lysate at 10 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution (purified) + Rat heart tissue lysate at 10 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Anti-CD36 antibody [EPR6573] (ab133625) at 1/10000 dilution (purified) + NIH/3T3 cell lysate at 10 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution (unpurified) + NIH/3T3 at 10 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
All lanes : Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution (unpurified)Lane 1 : 3T3-L1 cell lysateLane 2 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
ab133625 (unpurified) at 1/5 immunoprecipitating CD36 in 3T3-L1 cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.
ab133625 (purified) at 1/50 immunoprecipitating CD36 in 3T3-L1 cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.