![Overlay histogram showing THP1 cells stained with ab93941 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93941, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed THP1 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/24692_CD38-Primary-antibodies-ab93941-9.jpg)
Overlay histogram showing THP1 cells stained with ab93941 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93941, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed THP1 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
![Anti-CD38 antibody [6E12D] (ab93941) at 1/500 dilution + CD38-hIgGFc transfected HEK293 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/24690_CD38-Primary-antibodies-ab93941-1.jpg)
Anti-CD38 antibody [6E12D] (ab93941) at 1/500 dilution + CD38-hIgGFc transfected HEK293 cell lysate
Immunohistochemical analysis of paraffin embedded Human lung cancer (1) and lymphonodus tissue (2), showing cytomembrane localization using ab93941 with DAB staining.