ab2391 at a 1/100 dilution staining CD40L from human inflamed muscle tissue sections by immunohistochemistry (frozen sections). The tissue was acetone fixed (this provides a better result than unfixed tissue) and then incubated with the antibody for 24 hours at 4 °C. Alexa-Fluor ® 594 was used as the secondary antibody.See Abreview
ab2391 at 1/500 staining rat spleen tissue sections by IHC-P. The tissue was fomraldehyde fixed and blocked with serum prior to incubation with the antibody for 18 hours. An Alexa-Fluor ® 488 conjugated goat anti-rabbit was used as the secondary.See Abreview
ab2391 staining CD40L in Human platelet cells by Flow cytometry.Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/200 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.See Abreview