Anti-CD47 antibody [B6H12.2]

• Supplier: Abcam
• Catalog: ab3283
• Prices: $401.00
• Sizes: 500 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: B6H12.2
• Applications: FC FA ICC/IF IP ICC/IF ICC/IF IHC-F WB
• Species Reactivities: Human
• Antigen: Intact CD47 purified from placenta
• Description: Mouse Monoclonal
• Gene: CD47
• Conjugate: Unconjugated

Product images

ab3283 staining CD47 in HUVEC cells by Immunocytochemistry/ Immunofluorescence.The cells were fixed with 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.25% Triton X-100 for 5 minutes and blocked with 1% BSA in 0.1% Tween 20 and PBS for 1 hour at room temperature. The cells were incubated in a mixture of two primary antibodies ab3283 and a rabbit anti-human VEGFR2 overnight at 4 °C. The cells were then incubated with a mixture of two secondary antibodies (Alexa Fluor 488 anti-rabbit and Alexa Fluor 594 anti-mouse) in 1% BSA for 1 hour at room temperature in the dark. Counterstained with DAPI.

ab3283 at 1µg/ml staining CD47 from human OVCA3 cells by ICC. The cells were methanol fixed, blocked with 3% serum and incubated with the primary antibody for 30 minutes. A biotinylated horse anti-mouse IgG was used as the secondary antibody.See Abreview

Anti-CD47 antibody [B6H12.2] (ab3283) at 1 µg/ml + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Overlay histogram showing peripheral blood lymphocytes stained with ab3283 (red line). The cells were incubated with the antibody (ab3283, 1µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/200 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.

Product References

Antibody therapy targeting the CD47 protein is effective in a model of aggressive - Antibody therapy targeting the CD47 protein is effective in a model of aggressive

Edris B, Weiskopf K, Volkmer AK, Volkmer JP, Willingham SB, Contreras-Trujillo H, Liu J, Majeti R, West RB, Fletcher JA, Beck AH, Weissman IL, van de Rijn M. Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6656-61. doi:

The CD47-signal regulatory protein alpha (SIRPa) interaction is a therapeutic - The CD47-signal regulatory protein alpha (SIRPa) interaction is a therapeutic

Willingham SB, Volkmer JP, Gentles AJ, Sahoo D, Dalerba P, Mitra SS, Wang J, Contreras-Trujillo H, Martin R, Cohen JD, Lovelace P, Scheeren FA, Chao MP, Weiskopf K, Tang C, Volkmer AK, Naik TJ, Storm TA, Mosley AR, Edris B, Schmid SM, Sun CK, Chua MS, Murillo O, Rajendran P, Cha AC, Chin RK, Kim D, Adorno M, Raveh T, Tseng D, Jaiswal S, Enger PO, Steinberg GK, Li G, So SK, Majeti R, Harsh GR, van de Rijn M, Teng NN, Sunwoo JB, Alizadeh AA, Clarke MF, Weissman IL. Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6662-7. doi:

Heparan sulfate modification of the transmembrane receptor CD47 is necessary for - Heparan sulfate modification of the transmembrane receptor CD47 is necessary for

Kaur S, Kuznetsova SA, Pendrak ML, Sipes JM, Romeo MJ, Li Z, Zhang L, Roberts DD. J Biol Chem. 2011 Apr 29;286(17):14991-5002.

H-ras up-regulates expression of BNIP3. - H-ras up-regulates expression of BNIP3.

Kalas W, Swiderek E, Rapak A, Kopij M, Rak J, Strzadala L. Anticancer Res. 2011 Sep;31(9):2869-75.

Thrombospondin-1 inhibits VEGF receptor-2 signaling by disrupting its association - Thrombospondin-1 inhibits VEGF receptor-2 signaling by disrupting its association

Kaur S, Martin-Manso G, Pendrak ML, Garfield SH, Isenberg JS, Roberts DD. J Biol Chem. 2010 Dec 10;285(50):38923-32.

Amyloid-beta inhibits No-cGMP signaling in a CD36- and CD47-dependent manner. - Amyloid-beta inhibits No-cGMP signaling in a CD36- and CD47-dependent manner.

Miller TW, Isenberg JS, Shih HB, Wang Y, Roberts DD. PLoS One. 2010 Dec 22;5(12):e15686.

Regulation of cross-linked actin network (CLAN) formation in human trabecular - Regulation of cross-linked actin network (CLAN) formation in human trabecular

Filla MS, Schwinn MK, Sheibani N, Kaufman PL, Peters DM. Invest Ophthalmol Vis Sci. 2009 Dec;50(12):5723-31.

Interaction between Src homology 2 domain bearing protein tyrosine phosphatase - Interaction between Src homology 2 domain bearing protein tyrosine phosphatase

Yoshida H, Tomiyama Y, Oritani K, Murayama Y, Ishikawa J, Kato H, Miyagawa Ji J, Honma N, Nishiura T, Matsuzawa Y. J Immunol. 2002 Apr 1;168(7):3213-20.

Bidirectional negative regulation of human T and dendritic cells by CD47 and its - Bidirectional negative regulation of human T and dendritic cells by CD47 and its

Latour S, Tanaka H, Demeure C, Mateo V, Rubio M, Brown EJ, Maliszewski C, Lindberg FP, Oldenborg A, Ullrich A, Delespesse G, Sarfati M. J Immunol. 2001 Sep 1;167(5):2547-54.

Subversion of monocyte functions by coxiella burnetii: impairment of the - Subversion of monocyte functions by coxiella burnetii: impairment of the

Capo C, Lindberg FP, Meconi S, Zaffran Y, Tardei G, Brown EJ, Raoult D, Mege JL. J Immunol. 1999 Dec 1;163(11):6078-85.