CD47

Gene Symbol CD47
Entrez Gene 961
Alt Symbol IAP, MER6, OA3
Species Human
Gene Type protein-coding
Description CD47 molecule
Other Description CD47 antigen (Rh-related antigen, integrin-associated signal transducer)|CD47 glycoprotein|Rh-related antigen|antigen identified by monoclonal antibody 1D8|antigenic surface determinant protein OA3|integrin associated protein|integrin-associated protein|integrin-associated signal transducer|leukocyte surface antigen CD47
Swissprots D3DN59 Q08722 Q53Y71 Q96A60 A8K198
Accessions AAQ11189 EAW79732 EAW79733 EAW79734 Q08722 AI701980 AK096670 AK124152 AK289813 BAF82502 AL832675 BC010016 AAH10016 BC012884 AAH12884 BC037306 AAH37306 BC042889 BC045593 BC053959 BI753986 BT006907 AAP35553 BX640889 CB995865 DQ890756 ABM81682 DQ891811 ABM82737 DQ893920 ABM84846 LN680437 CEJ95640 X69398 CAA49196 Z25521 CAA80977 XM_005247908 XP_005247965 XM_005247909 XP_005247966 XR_241521 XR_241522 XR_924218 XR_924219 XR_924220 NM_001777 NP_001768 NM_198793 NP_942088 NM_001025079 NM_001025080
Function Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. {ECO:0000250, ECO:0000269|PubMed:11509594, ECO:0000269|PubMed:15383453, ECO:0000269|PubMed:7691831}.
Subcellular Location Cell membrane {ECO:0000269|PubMed:7691831}; Multi-pass membrane protein {ECO:0000269|PubMed:7691831}.
Tissue Specificity Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
Top Pathways ECM-receptor interaction

CD47 (C-18) - sc-7057 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CD47 (C-18): sc-7057. Western blot analysis of CD47 expression in HeLa (A), ECV304 (B) and HISM (C) whole cell lysates.

CD47 (H-100) - sc-25773 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CD47 (H-100): sc-25773. Western blot analysis of CD47 expression in human platelet extract.

CD47 (B6H12) - sc-12730 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD47 (B6H12): sc-12730: Western blot analysis of CD47 expression in non-transfected HEK 293T (A) and puromycin treated, CD47 CRISPR/Cas9 KO Plasmid (h): sc-400508 plus CD47 HDR Plasmid (h): sc-400508-HDR co-transfected HEK 293T (B) whole cell lysates. β-Actin (C4): sc-47778 used as specificity and loading control.

CD47 (S-19) - sc-7059 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CD47 (S-19): sc-7059. Western blot analysis of CD47 expression in HeLa (A), Jurkat (B), BYDP (C) and ECV304 (D) whole cell lysates.

CD47 (CC2C6) - sc-21786 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD47 (CC2C6) PE: sc-21786 PE. FCM analysis of human peripheral blood leukocytes. Black line histogram represents the isotype control, normal mouse IgG<sub>1</sub>: sc-2866.

CD47 (BRIC 126) - sc-59079 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD47 (BRIC 126): sc-59079. Western blot analysis of CD47 expression in non-transfected: sc-110760 (A) and human CD47 transfected: sc-112975 (B) 293 whole cell lysates.

Anti-CD47 antibody - ab175388 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab175388. Blue DAPI for nuclear staining.

Anti-CD47 antibody - ab136550 from Abcam

Host Rabbit
Clonality Polyclonal
ICC/IF image of ab136550 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab136550 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CD47 antibody - ab193940 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
All lanes : Anti-CD47 antibody (ab193940) at 0.3 µg/mlLane 1 : Human hippocampus lysate (in RIPA buffer)Lane 2 : HeLa lysate (in RIPA buffer)Lysates/proteins at 35 µg per lane.developed using the ECL technique

Anti-CD47 antibody - ab192827 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-CD47 antibody (ab192827) at 1/1000 dilution + Jurkat cell lysate

Anti-CD47 antibody - ab118222 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-CD47 antibody (ab118222) at 1/1000 dilution + Jurkat cell lysate

Anti-CD47 antibody - ab171767 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-CD47 antibody (ab171767) at 1 µg/mlLane 1 : CD47 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.

Anti-CD47 antibody [B6H12], prediluted (Allophycocyanin) - ab134485 from Abcam

Prices $188.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone B6H12
Flow cytometric analysis of Human PBMC labelling CD47 with Mouse IgG1K Isotype control APC (blue histogram) or ab134485 (purple histogram). Cells in the lymphocyte gate were used for analysis.

Anti-CD47 antibody [B6H12], prediluted (FITC) - ab134484 from Abcam

Prices $188.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone B6H12
Flow cytometric analysis of Human PBMC labelling CD47 with Mouse IgG1K Isotype control FITC (open histogram) or ab134484 (filled histogram). Cells in the lymphocyte gate were used for analysis.

Anti-CD47 antibody [B6H12.2] - ab3283 from Abcam

Prices $401.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone B6H12.2
ab3283 staining CD47 in HUVEC cells by Immunocytochemistry/ Immunofluorescence.The cells were fixed with 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.25% Triton X-100 for 5 minutes and blocked with 1% BSA in 0.1% Tween 20 and PBS for 1 hour at room temperature. The cells were incubated in a mixture of two primary antibodies ab3283 and a rabbit anti-human VEGFR2 overnight at 4 °C. The cells were then incubated with a mixture of two secondary antibodies (Alexa Fluor 488 anti-rabbit and Alexa Fluor 594 anti-mouse) in 1% BSA for 1 hour at room temperature in the dark. Counterstained with DAPI.

Anti-CD47 antibody [EPR4150(2)] - ab108415 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4150(2)
All lanes : Anti-CD47 antibody [EPR4150(2)] (ab108415) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : K562 cell lysateLysates/proteins at 10 µg per lane.

Anti-CD47 antibody [EPR4150(2)] (HRP) - ab199520 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4150(2)
All lanes : Anti-CD47 antibody [EPR4150(2)] (HRP) (ab199520) at 1/5000 dilutionLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-CD47 antibody [MEM-122] - ab9089 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MEM-122
Human peripheral blood lymphocytes stained with ab9089 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab9089, 0.1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral bloo

Anti-CD47 antibody - C-terminal - ab176099 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-CD47 antibody - C-terminal (ab176099) at 1/1000 dilution + T47D cell line lysate at 35 µg

Anti-CD47 Antibody (aa19-158) - LS-C150168 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-CD47 Antibody (clone 1/1A4) - LS-C188327 from LifeSpan Bioscience

Prices $250.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1/1A4

Anti-CD47 Antibody (clone 1/1A4, Low Endotoxin) - LS-C188326 from LifeSpan Bioscience

Prices $445.00
Sizes 500 µg
Host Mouse
Clonality Monoclonal
Clone 1/1A4

Anti-CD47 Antibody (clone B6H12.2, PE) - LS-C148707 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone B6H12.2
Cell surface flow cytometry of CD47 in 5 x 10^5 human PBMCs using 10 ul of LS-C148707. Green histogram represents isotype control, ; red represents CD47 antibody.

Anti-CD47 Antibody (C-Terminus, AP) - LS-C254068 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CD47 Antibody (clone BRIC126) - LS-C202089 from LifeSpan Bioscience

Prices $465.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal
Clone BRIC126
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