Anti-CD55 antibody [MEM-118]
| Name | Anti-CD55 antibody [MEM-118] |
|---|---|
| Supplier | Abcam |
| Catalog | ab1422 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgM |
| Clone | MEM-118 |
| Applications | IHC-P FC IP ICC/IF ICC/IF |
| Species Reactivities | Human, Primate |
| Antigen | Tissue/ cell preparation (Human) |
| Description | Mouse Monoclonal |
| Gene | CD55 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Human peripheral blood lymphocytes stained with ab1422 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab1422, 1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/25321_ab1422-1-ab1422FC.jpg)
Human peripheral blood lymphocytes stained with ab1422 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab1422, 1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood
Product References
Exploitation of the complement system by oncogenic Kaposi's sarcoma-associated - Exploitation of the complement system by oncogenic Kaposi's sarcoma-associated
Lee MS, Jones T, Song DY, Jang JH, Jung JU, Gao SJ. PLoS Pathog. 2014 Sep 25;10(9):e1004412.
The nature of large noncovalent complexes containing - The nature of large noncovalent complexes containing
Cinek T, Horejsi V. J Immunol. 1992 Oct 1;149(7):2262-70.