CD55

Gene Symbol CD55
Entrez Gene 1604
Alt Symbol CR, CROM, DAF, TC
Species Human
Gene Type protein-coding
Description CD55 molecule, decay accelerating factor for complement (Cromer blood group)
Other Description CD55 antigen|complement decay-accelerating factor
Swissprots D3DT84 P78361 Q14UF3 B1AP14 P09679 Q14UF4 D3DT83 Q14UF2 Q14UF5 E7ER69 Q14UF6 P08174
Accessions AAA52170 AAC60633 AAW29942 BAA22900 CBX47421 EAW93485 EAW93486 EAW93487 EAW93488 EAW93489 EAW93490 EAW93491 EAW93492 EAW93493 EAW93494 EAW93495 EAW93496 EAW93497 EAW93498 EAW93499 P08174 AB240566 BAE97422 AB240567 BAE97423 AB240568 BAE97424 AB240569 BAE97425 AB240570 BAE97426 AF052110 AK298549 BAG60746 AK300621 BAG62314 AK312448 BAG35355 AW316643 AY055757 AAL25832 AY055758 AAL25833 AY055759 AAL25834 AY055760 AAL25835 BC001288 AAH01288 BI545505 BT007159 AAP35823 CA432400 CA748776 DC386415 DQ890776 ABM81702 DQ893935 ABM84861 M15799 AAA52167 M30142 AAA52168 M31516 AAA52169 S70688 AAB20576 U88576 AAB48622 NM_000574 NP_000565 NM_001114752 NP_001108224 NM_001300902 NP_001287831 NM_001300903 NP_001287832 NM_001300904 NP_001287833 NR_125349 NM_001114543 NM_001114544
Function This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. {ECO:0000269|PubMed:7525274}.
Subcellular Location Isoform 7: Cell membrane {ECO:0000305|PubMed:16503113}; Lipid-anchor, GPI-anchor {ECO:0000305|PubMed:16503113}.
Tissue Specificity Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
Top Pathways Complement and coagulation cascades, Hematopoietic cell lineage

CD55 (143-30) - sc-21769 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD55 (143-30): sc-21769. Western blot analysis CD55 expression in AML-193 whole cell lysate under non-reducing conditions.

CD55 (H-319) - sc-9156 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CD55 (H-319): sc-9156. Western blot analysis of CD55 expression in non-transfected: sc-110760 (A) and human CD55 transfected: sc-110477 (B) 293 whole cell lysates.

CD55 (67) - sc-53207 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD55 (67): sc-53207. Western blot analysis of CD55 expression in AML-193 whole cell lysate.

CD55 (NaM16-4D3) - sc-51733 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD55 (NaM16-4D3): sc-51733. Western blot analysis of CD55 expression in non-transfected: sc-110760 (A) and human CD55 transfected: sc-110477 (B) 293 whole cell lysates.

CD55 (H-7) - sc-133220 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD55 (H-7): sc-133220. Western blot analysis of CD55 expression in non-transfected: sc-110760 (A) and human CD55 transfected: sc-110477 (B) 293 whole cell lysates.

CD55 (BRIC 216) - sc-59092 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD55 (BRIC 216): sc-59092. Indirect FCM analysis of human peripheral blood leukocytes stained with CD55 (BRIC 216), followed by PE-conjugated goat anti-mouse IgG: sc-3738. Black line histogram represents the isotype control, normal mouse IgG1: sc-3877

Anti-CD55 antibody - ab96680 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-CD55 antibody (ab96680) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

Anti-CD55 antibody - ab54595 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
IHC image of CD55 staining in Human Lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab54595, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CD55 antibody - ab126221 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-CD55 antibody (ab126221) at 1/3000 dilution + K562 whole cell lysate at 30 µg

Anti-CD55 antibody - ab136145 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-CD55 antibody (ab136145) at 1/500 dilution + 293 cell lysate at 30 µg

Anti-CD55 antibody - ab37720 from Abcam

Prices $376.00
Sizes 50 µg
Host Chicken
Clonality Polyclonal
Anti-CD55 antibody (ab37720) at 1/2000 dilution + E. coli-derived fusion protein at 2.5 µgSecondaryGoat anti-IgY-HRP. at 1/1000 dilution

Anti-CD55 antibody [143-30] - ab95522 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 143-30

Anti-CD55 antibody [143-30] (Biotin) - ab82596 from Abcam

Prices $370.00
Sizes 100 tests
Host Mouse
Clonality Monoclonal
Clone 143-30

Anti-CD55 antibody [143-30] (FITC) - ab25634 from Abcam

Prices $193.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone 143-30
Overlay histogram showing peripheral blood lymphocytes stained with ab25634 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25634, 0.5 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.

Anti-CD55 antibody [143-30] (PE/Cy5®) - ab25410 from Abcam

Prices $370.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone 143-30

Anti-CD55 antibody [143-30] (Phycoerythrin) - ab25540 from Abcam

Prices $185.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone 143-30

Anti-CD55 antibody [67] - ab20145 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 67

Anti-CD55 antibody [BRIC 216] - ab33111 from Abcam

Prices $373.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone BRIC 216

Anti-CD55 antibody [EPR6689] - ab133684 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6689
All lanes : Anti-CD55 antibody [EPR6689] (ab133684) at 1/10000 dilutionLane 1 : A594 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-CD55 antibody [MEM-118] - ab1422 from Abcam

Prices $379.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MEM-118
Human peripheral blood lymphocytes stained with ab1422 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab1422, 1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood

Anti-CD55 antibody [MEM-118] (Alexa Fluor® 488) - ab187775 from Abcam

Prices $370.00
Sizes 100 tests
Host Mouse
Clonality Monoclonal
Clone MEM-118

Anti-CD55 antibody [MEM-118] (Biotin) - ab26005 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MEM-118

Anti-CD55 antibody [MEM-118] (Phycoerythrin) - ab130421 from Abcam

Prices $370.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone MEM-118

Anti-CD55 antibody [MEM-118], prediluted (FITC) - ab28112 from Abcam

Prices $376.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone MEM-118
Overlay histogram showing THP1 cells stained with ab28112 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28112, 10µl/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM (FITC) (ab91546, 10µl/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-CD55 antibody [MM0175-12L29] - ab89190 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone MM0175-12L29
IHC image of CD55 staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89190, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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