All lanes : Anti-CD68 antibody (ab63896) at 1 µg/mlLane 1 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 2 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 3 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 5 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLane 7 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate (ab7913)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.