CD68

Gene Symbol CD68
Entrez Gene 968
Alt Symbol GP110, LAMP4, SCARD1
Species Human
Gene Type protein-coding
Description CD68 molecule
Other Description CD68 antigen|macrophage antigen CD68|macrosialin|scavenger receptor class D, member 1
Swissprots P34810 Q53XI3 Q53HR6 Q96BI7 B4DVT4
Accessions AAC70006 EAW90165 P34810 AK222492 BAD96212 AK222514 BAD96234 AK290238 BAF82927 AK298055 BAG60349 AK301223 BAG62796 AW294871 BC015557 AAH15557 BT009923 AAP88925 BX438711 BX647524 DB187273 DQ890631 ABM92248 DQ893801 ABM84727 S57235 AAB25811 NM_001040059 NP_001035148 NM_001251 NP_001242
Function Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Subcellular Location Isoform Short: Cell membrane; Single-pass type I membrane protein.
Tissue Specificity Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. {ECO:0000269|PubMed:18405323}.
Top Pathways Lysosome

CD68 (C-9) - sc-393951 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD68 (C-9): sc-393951. Western blot analysis of CD68 expression in THP-1 (A), U-937 (B) and AML-193 (C) whole cell lysates.

CD68 (C-18) - sc-7082 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CD68 (C-18): sc-7082. Western blot analysis of human recombinant CD68 fusion protein.

CD68 (E-11) - sc-17832 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD68 (E-11): sc-17832. Immunofluorescence staining of methanol-fixed THP-1 cells showing cytoplasmic and membrane localization.

CD68 (KP1) - sc-20060 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD68 (KP1): sc-20060. Immunoperoxidase staining of formalin-fixed paraffin embedded human coronary artery tissue showing macrophage staining adjacent to the lumina (A,B). Kindly provided by Dr. John Sanders, University of Virginia.

CD68 (H-255) - sc-9139 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CD68 (H-255): sc-9139. Immunofluorescence staining of methanol-fixed THP-1 cells showing cytoplasmic and membrane localization.

CD68 (N-19) - sc-7083 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CD68 (N-19): sc-7083. Western blot analysis of CD68 expression in THP-1 whole cell lysate.

CD68 (SPM130) - sc-52998 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD68 (SPM130): sc-52998. Immunoperoxidase staining of formalin-fixed paraffin embedded human coronary artery tissue showing macrophage staining adjacent to the lumina (A,B). Kindly provided by Dr. John Sanders, University of Virginia.

CD68 (3F103) - sc-70761 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD68 (3F103): sc-70761. Western blot analysis of CD68 expression in THP-1 whole cell lysate.

Anti-CD68 antibody - ab125047 from Abcam

Prices $388.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal
ab125047 staining CD68 in Mouse vasculature tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 5 minutes at 20°C; antigen retrieval was by heat mediation in a tris buffer. Samples were incubated with primary antibody (1/100 in antibody diluent) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.See Abreview

Anti-CD68 antibody - ab63896 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CD68 antibody (ab63896) at 1 µg/mlLane 1 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 2 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 3 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 5 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLane 7 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate (ab7913)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-CD68 antibody - ab125475 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-CD68 antibody (ab125475) at 1 µg/ml + Jurkat cell lysate at 10 µg

Anti-CD68 antibody - ab125157 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Staining of CD68 in Paraffin-embedded Human tonsil tissue by Immunohistochemistry using ab125157 at a dilution of 1/100.

Anti-CD68 antibody - ab74208 from Abcam

Prices $376.00
Sizes 200 µg
Host Rabbit
Clonality Polyclonal
Anti-CD68 antibody (ab74208) at 1/25 dilution + Human Brain lysate

Anti-CD68 antibody [3F7D3] - ab201973 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F7D3
Anti-CD68 antibody [3F7D3] (ab201973) at 1/500 dilution + Human CD68 (aa42-155) recombinant protein

Anti-CD68 antibody [514H12] - ab49777 from Abcam

Prices $382.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal
Clone 514H12
This image shows human tonsil tissue stained with Ab 49777 at 1/40 dilution. Note intense cytoplasmic staining of macrophages.

Anti-CD68 antibody [C68/684] - ab201340 from Abcam

Prices $359.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone C68/684
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD68 with ab201340 at 1 µg/ml.

Anti-CD68 antibody [EPR1392Y] - ab76308 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1392Y
All lanes : Anti-CD68 antibody [EPR1392Y] (ab76308) at 1/2000 dilution (purified)Lane 1 : L292 cell lysateLane 2 : Neuro-2a cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-CD68 antibody [KP1] - ab955 from Abcam

Prices $394.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone KP1
ab955 staining CD68 in Human ulcerated Oral (Mucosa/Bone) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton-X 100 in PBS and blocked with 2.5% serum for 90 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were incubated with primary antibody (1/500 in 1% serum in PBS +0.01% Triton-X 100) for 16 hours at 4°C. A commercial IHC kit and DAB was used to visualize the staining.See Abreview

Anti-CD68 antibody [KP1 + C68/684] - ab199000 from Abcam

Prices $359.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone KP1 + C68/684
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD68 with ab199000 at 1µg/ml (10X).

Anti-CD68 antibody [KP1], prediluted - ab845 from Abcam

Prices $384.00
Sizes 6 ml
Host Mouse
Clonality Monoclonal
Clone KP1
Overlay histogram showing THP1 cells stained with ab845 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab845, 1/4 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-CD68 antibody [KP1], prediluted - ab74704 from Abcam

Prices $382.00
Sizes 7 ml
Host Mouse
Clonality Monoclonal
Clone KP1
Formalin-fixed, paraffin-embedded human tonsil stained with ab74704 using peroxidase-conjugate and AEC chromogen. Note intense cytoplasmic staining of macrophages.

Anti-CD68 antibody [PG-M1] - ab783 from Abcam

Prices $376.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone PG-M1

Anti-CD68 antibody [PG-M1], prediluted - ab844 from Abcam

Prices $376.00
Sizes 6 ml
Host Mouse
Clonality Monoclonal
Clone PG-M1
ab844 staining CD68 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry. Staining was detected using DAB.

Anti-CD68 antibody [Y1/82A] - ab134350 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone Y1/82A
Intracellular immunofluorescent staining with flow cytometric analysis labelling CD68 in Human peripheral blood monocytes at 0.125 µg per million cells in 100 µl volume, intracellularly stained by ab134351.

Anti-CD68 antibody [Y1/82A] (Biotin) - ab95564 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone Y1/82A
Flow cytometry staining of normal human peripheral blood cells with Anti-Mouse CD11b FITC and 0.06 µg of Mouse IgG2b ? Isotype Control Biotin (left) or 0.06 µg of ab95564 (right) followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.
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