Anti-CD97 antibody [EPR4427]

• Supplier: Abcam
• Catalog: ab108368
• Prices: $388.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4427
• Applications: WB IP IHC-P ICC/IF ICC/IF ICC/IF
• Species Reactivities: Mouse, Human
• Antigen: A synthetic peptide corresponding to residues in the extracellular domain of Human CD97
• Description: Rabbit Monoclonal
• Gene: ADGRE5
• Conjugate: Unconjugated

Product images

ICC/IF image of ab108368 stained MDA-MB-231 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108368 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

All lanes : Anti-CD97 antibody [EPR4427] (ab108368) at 1/1000 dilutionLane 1 : Human tonsil lysateLane 2 : Jurkat cell lysateLane 3 : U937 cell lysateLane 4 : Human fetal thymus lysateLysates/proteins at 10 µg per lane.

ab108368 at 1/250 dilution staining CD97 in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.

ab108368 at 1/250 dilution staining CD97 in Human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.

ab108368 at 1/250 dilution staining CD97 in Human colonic tonsil by Immunohistochemistry, Paraffin-embedded tissue.

Product References

Overexpression of CD97 confers an invasive phenotype in glioblastoma cells and is - Overexpression of CD97 confers an invasive phenotype in glioblastoma cells and is

Safaee M, Clark AJ, Oh MC, Ivan ME, Bloch O, Kaur G, Sun MZ, Kim JM, Oh T, Berger MS, Parsa AT. PLoS One. 2013 Apr 26;8(4):e62765.