All lanes : Anti-Cdc23 antibody [EPR11827] (ab177148) at 1/10000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : K562 cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunofluorescent analysis of HeLa cells labeling Cdc23 with ab177148 at 1/50 dilution.
Western blot analysis on immunoprecipitation pellet from (Lane 1) K562 cells lysate or (Lane 2) 1X PBS (negative control) using ab177148 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.