Immunofluorescent analysis of acetone-fixed HeLa cells labeling Cdc23 with ab182003 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution (Left panel). Counter stained with Dapi (Right panel).
All lanes : Anti-Cdc23 antibody [EPR11828] (ab182003) at 1/10000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : K562 cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution