Anti-Cdc4 / Fbw7 / hSel 10 antibody

• Supplier: Abcam
• Catalog: ab12292
• Prices: $390.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF IP
• Species Reactivities: Human, Mouse
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Cdc4/ Fbw7/ hSel 10
• Blocking Peptide: Human Cdc4 / Fbw7 / hSel 10 peptide
• Description: Rabbit Polyclonal
• Gene: FBXW7
• Conjugate: Unconjugated

Product images

ab12292 (1/2000) detecting Cdc4 in Hela cells (green). Cells were fixed in methanol, permeabilised with 0.5% Triton X100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.See Abreview

ICC/IF image of ab12292 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12292, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, HepG2 and MCF7 cells at 5µg/ml.

All lanes : Anti-Cdc4 / Fbw7 / hSel 10 antibody (ab12292) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear LysateLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Product References

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Liu N, Li H, Li S, Shen M, Xiao N, Chen Y, Wang Y, Wang W, Wang R, Wang Q, Sun J, Wang P. J Biol Chem. 2010 Jun 11;285(24):18858-67.

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Lan K, Verma SC, Murakami M, Bajaj B, Kaul R, Robertson ES. Proc Natl Acad Sci U S A. 2007 Oct 9;104(41):16287-92. Epub 2007 Oct 1.