FBXW7

Gene Symbol FBXW7
Entrez Gene 55294
Alt Symbol AGO, CDC4, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4
Species Human
Gene Type protein-coding
Description F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase
Other Description F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila)|F-box protein FBW7|F-box protein FBX30|F-box protein SEL-10|F-box/WD repeat-containing protein 7|archipelago|homolog of C elegans sel-10
Swissprots Q96RI2 Q68DR0 Q9NUX6 Q96LE0 Q969H0 Q96A16 B7ZLP9
Accessions AFD64626 AFD64627 BAF73721 EAX04977 EAX04978 EAX04979 Q969H0 AB209725 BAD92962 AF383178 AAK60269 AF411971 AAL06290 AF411972 AAL06291 AI598129 AK001933 BAA91986 AK131383 AK223449 BAD97169 AK294568 BAH11814 AK299722 BAH13109 AK315142 AW965611 AY008274 AAG16640 AY033553 AAK57547 AY049984 AAL07271 BC014503 BC037320 AAH37320 BC059373 BC117244 AAI17245 BC117246 AAI17247 BC143944 AAI43945 BF514288 BI496455 BX498391 CR749305 CAH18160 DA321362 HQ258348 ADR83102 HQ873864 AEK48270 HQ873865 AEK48271 HQ873866 AEK48272 HQ873867 AEK48273 HQ873868 AEK48274 HQ873869 AEK48275 HQ873870 AEK48276 W39194 XM_011532083 XP_011530385 XM_011532084 XP_011530386 XM_011532085 XP_011530387 XM_011532086 XP_011530388 XM_011532087 XP_011530389 XM_011532088 XP_011530390 NM_001013415 NP_001013433 NM_001257069 NP_001243998 NM_018315 NP_060785 NM_033632 NP_361014
Function Substrate recognition component of an SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (PubMed:17434132). Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1 (PubMed:11565034, PubMed:12354302, PubMed:11585921, PubMed:15103331, PubMed:14739463, PubMed:17558397, PubMed:17873522, PubMed:22608923). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (PubMed:14739463). {ECO:0000269|PubMed:11565034, ECO:0000269|PubMed:11585921, ECO:0000269|PubMed:14739463, ECO:0000269|PubMed:15103331, ECO:0000269|PubMed:17434132, ECO:0000269|PubMed:17558397, ECO:0000269|PubMed:17873522, EC
Subcellular Location Isoform 3: Nucleus, nucleolus {ECO:0000269|PubMed:17558397}.
Tissue Specificity Isoform 1 is widely expressed. Isoform 3 is expressed in brain. {ECO:0000269|PubMed:12354302}.
Top Pathways Ubiquitin mediated proteolysis

Cdc4 (H-300) - sc-33196 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cdc4 (H-300): sc-33196. Western blot analysis of human recombinant Cdc4 fusion protein (A) and Jurkat whole cell lysate (B).

Cdc4 (D-16) - sc-21184 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Cdc4 (D-16): sc-21184. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic and nuclear staining of exocrine glandular cells and Islets of Langerhans.

Cdc4 (N-17) - sc-21185 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Cdc4 (X-23) - sc-133448 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
Cdc4 (X-23): sc-133448. Western blot analysis of Cdc4 expression in Jurkat whole cell lysate.

Anti-Cdc4 / Fbw7 / hSel 10 antibody - ab12292 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Cdc4 / Fbw7 / hSel 10 antibody - ab55036 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Cdc4 / Fbw7 / hSel 10 antibody (ab55036) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lung.

Anti-Cdc4 / Fbw7 / hSel 10 antibody - ab47242 from Abcam

Prices $372.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Cdc4 / Fbw7 / hSel 10 antibody [FBOX 3a9/1] - ab74054 from Abcam

Prices $391.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone FBOX 3a9/1
ab74054 at 1/100 dilution staining Cdc4 / Fbw7 / hSel 10 in human Hela cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed with 4% paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 1% bovine serum albumin in PBS for 1 h. A FITC-conjugated anti-mouse secondary antibody was used at 1/100 dilution. The nuclei were stained with 0.5 μg/ml 4′,6-diamidino-2-phenylindole. A mouse IgG was used as isotype control from other source. 

Anti-Fbxw7 antibody - ab109617 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Fbxw7 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to Fbxw7 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab109617.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 69kDa; Fbxw7

Anti-Fbxw7 antibody - ab168427 from Abcam

Host Mouse
Clonality Polyclonal
Immunofluorescence analysis of HeLa cells labeling Fbxw7 with ab168427 at 10&microg/ml.

Anti-Fbxw7 antibody - ab192328 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Fbxw7 antibody - ab154663 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Fbxw7 antibody (ab154663) at 1/1000 dilution + Ragi whole cell lysate at 30 µg

Anti-Fbxw7 antibody - ab84783 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast carcinoma, using 1/500 ab84783 followed by DAB staining.

Anti-Fbxw7 antibody - ab81256 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Fbxw7 antibody [3D1] - ab128062 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 3D1
ab128062, at 5 µg/ml, staining Fbxw7 in paraffin-embedded Human liver tissue by Immunohistochemistry.

Anti-Fbxw7 antibody [4C11] - ab180472 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4C11
All lanes : Anti-Fbxw7 antibody [4C11] (ab180472) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Fbxw7 cDNALysates/proteins at 5 µg per lane.

Anti-Fbxw7 antibody [EPR8069(2)] - ab171961 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8069(2)
All lanes : Anti-Fbxw7 antibody [EPR8069(2)] (ab171961) at 1/1000 dilutionLane 1 : Human fetal heart lysateLane 2 : Human fetal muscle lysateLane 3 : Human small intestine lysateLysates/proteins at 10 µg per lane.

Anti-Fbxw7 antibody [FBOX 3a9/1] (FITC) - ab187816 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone FBOX 3a9/1

Anti-Fbxw7 antibody [FBOX 3a9/1] (HRP) - ab187815 from Abcam

Prices $372.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone FBOX 3a9/1

Anti-Fbxw7 antibody - C-terminal - ab198238 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Fbxw7 with ab198238 at 1/40 dilution.

Anti-FBXW7 / FBW7 Antibody (aa1-354, clone 4C11) - LS-C336721 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4C11
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FBXW7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Anti-FBXW7 / FBW7 Antibody (C-Terminus) IHC-plus™ - LS-B5800 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-FBXW7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5800 concentration 2.5 ug/ml.

Anti-FBXW7 / FBW7 Antibody (aa251-300) IHC-plus™ - LS-B5634 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-FBXW7 antibody IHC of mouse brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5634 concentration 5 ug/ml.

Anti-FBXW7 / FBW7 Antibody (aa1-354, clone 13H4) - LS-C336747 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 13H4
IHC of paraffin-embedded Human tonsil using anti-FBXW7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Anti-FBXW7 / FBW7 Antibody (aa1-354, clone 2G7) - LS-C336704 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2G7
IHC of paraffin-embedded Human tonsil using anti-FBXW7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
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