Anti-Cdc4 / Fbw7 / hSel 10 antibody [FBOX 3a9/1]
| Name | Anti-Cdc4 / Fbw7 / hSel 10 antibody [FBOX 3a9/1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab74054 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | FBOX 3a9/1 |
| Applications | WB IP IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide corresponding to Human Cdc4/ Fbw7/ hSel 10 |
| Description | Mouse Monoclonal |
| Gene | FBXW7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab74054 at 1/100 dilution staining Cdc4 / Fbw7 / hSel 10 in human Hela cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed with 4% paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 1% bovine serum albumin in PBS for 1 h. A FITC-conjugated anti-mouse secondary antibody was used at 1/100 dilution. The nuclei were stained with 0.5 μg/ml 4′,6-diamidino-2-phenylindole. A mouse IgG was used as isotype control from other source.
![All lanes : Anti-Cdc4 / Fbw7 / hSel 10 antibody [FBOX 3a9/1] (ab74054) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/26370_Cdc4-Fbw7-hSel-10-Primary-antibodies-ab74054-3.jpg)
All lanes : Anti-Cdc4 / Fbw7 / hSel 10 antibody [FBOX 3a9/1] (ab74054) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Overlay histogram showing HeLa cells stained with ab74054 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab74054, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/26371_Cdc4-Fbw7-hSel-10-Primary-antibodies-ab74054-4.jpg)
Overlay histogram showing HeLa cells stained with ab74054 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab74054, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Prognostic significance of Fbw7 in human melanoma and its role in cell migration. - Prognostic significance of Fbw7 in human melanoma and its role in cell migration.
Cheng Y, Chen G, Martinka M, Ho V, Li G. J Invest Dermatol. 2013 Jul;133(7):1794-802.
Recombinant human adenovirus-p53 injection induced apoptosis in hepatocellular - Recombinant human adenovirus-p53 injection induced apoptosis in hepatocellular
Tu K, Zheng X, Zhou Z, Li C, Zhang J, Gao J, Yao Y, Liu Q. PLoS One. 2013 Jul 1;8(7):e68574.
The Fbw7/human CDC4 tumor suppressor targets proproliferative factor KLF5 for - The Fbw7/human CDC4 tumor suppressor targets proproliferative factor KLF5 for
Liu N, Li H, Li S, Shen M, Xiao N, Chen Y, Wang Y, Wang W, Wang R, Wang Q, Sun J, Wang P. J Biol Chem. 2010 Jun 11;285(24):18858-67.
The ubiquitin ligase SCFFbw7 antagonizes apoptotic JNK signaling. - The ubiquitin ligase SCFFbw7 antagonizes apoptotic JNK signaling.
Nateri AS, Riera-Sans L, Da Costa C, Behrens A. Science. 2004 Feb 27;303(5662):1374-8. Epub 2004 Jan 22.