Anti-CDC5L antibody
| Name | Anti-CDC5L antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab31779 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Human, Dog |
| Antigen | Synthetic peptide derived from within residues 750 to the C-terminus of Human CDC5L |
| Description | Rabbit Polyclonal |
| Gene | CDC5L |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-CDC5L antibody (ab31779) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab31779 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31779, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Image courtesy of Human Protein Atlas ab31779 staining in human stomach, showing staining of the glanular cells (in brown). Paraffin embedded lower stomach tissue was incubated with ab31779 (1:60 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.ab31779 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.
Product References
The RNA polymerase II C-terminal domain promotes splicing activation through - The RNA polymerase II C-terminal domain promotes splicing activation through
David CJ, Boyne AR, Millhouse SR, Manley JL. Genes Dev. 2011 May 1;25(9):972-83.
Interaction between antibody-diversification enzyme AID and - Interaction between antibody-diversification enzyme AID and
Conticello SG, Ganesh K, Xue K, Lu M, Rada C, Neuberger MS. Mol Cell. 2008 Aug 22;31(4):474-84.