Anti-CDCA5 antibody [EPR16331] - C-terminal

• Supplier: Abcam
• Catalog: ab192237
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16331
• Applications: FC ICC/IF ICC/IF WB IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CDCA5 aa 200 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: CDCA5
• Conjugate: Unconjugated

Product images

Flow cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling CDCA5 with ab192237 at 1/100 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling CDCA5 with ab192237 at 1/100 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).Negative controls: anti-CDCA5 at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.

Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling CDCA5 with ab192237 at 1/100 (5 µg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CDCA5 with ab192237 at 1/100 (5 µg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237) at 1/1000 dilution + SH-SY5Y cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

All lanes : Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237) at 1/10000 dilutionLane 1 : 293 cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution