Anti-CDCP1 antibody
| Name | Anti-CDCP1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab1377 |
| Prices | $380.00 |
| Sizes | 100 µg |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB IP |
| Species Reactivities | Human |
| Antigen | Synthetic peptide: PLLNTQEPMEPAE, corresponding to C terminal amino acids 824-836 of Human CDCP1 |
| Description | Goat Polyclonal |
| Gene | CDCP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab1377 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1377, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab1377 staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1377, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
CUB-domain containing protein 1 represses the epithelial phenotype of pancreatic - CUB-domain containing protein 1 represses the epithelial phenotype of pancreatic
Miura S, Hamada S, Masamune A, Satoh K, Shimosegawa T. Exp Cell Res. 2014 Feb 15;321(2):209-18.
ADAM9 promotes lung cancer metastases to brain by a plasminogen activator-based - ADAM9 promotes lung cancer metastases to brain by a plasminogen activator-based
Lin CY, Chen HJ, Huang CC, Lai LC, Lu TP, Tseng GC, Kuo TT, Kuok QY, Hsu JL, Sung SY, Hung MC, Sher YP. Cancer Res. 2014 Sep 15;74(18):5229-43.
CDCP1 regulates the function of MT1-MMP and invadopodia-mediated invasion of - CDCP1 regulates the function of MT1-MMP and invadopodia-mediated invasion of
Miyazawa Y, Uekita T, Ito Y, Seiki M, Yamaguchi H, Sakai R. Mol Cancer Res. 2013 Jun;11(6):628-37.
Prognostic significance of CUB domain containing protein expression in - Prognostic significance of CUB domain containing protein expression in
Mamat S, Ikeda J, Enomoto T, Ueda Y, Rahadiani N, Tian T, Wang Y, Qiu Y, Kimura T, Aozasa K, Morii E. Oncol Rep. 2010 May;23(5):1221-7.
Proteolysis-induced N-terminal ectodomain shedding of the integral membrane - Proteolysis-induced N-terminal ectodomain shedding of the integral membrane
He Y, Wortmann A, Burke LJ, Reid JC, Adams MN, Abdul-Jabbar I, Quigley JP, Leduc R, Kirchhofer D, Hooper JD. J Biol Chem. 2010 Aug 20;285(34):26162-73.
Expression of CUB domain containing protein (CDCP1) is correlated with prognosis - Expression of CUB domain containing protein (CDCP1) is correlated with prognosis
Ikeda J, Oda T, Inoue M, Uekita T, Sakai R, Okumura M, Aozasa K, Morii E. Cancer Sci. 2009 Mar;100(3):429-33.
Functional role of cell surface CUB domain-containing protein 1 in tumor cell - Functional role of cell surface CUB domain-containing protein 1 in tumor cell
Deryugina EI, Conn EM, Wortmann A, Partridge JJ, Kupriyanova TA, Ardi VC, Hooper JD, Quigley JP. Mol Cancer Res. 2009 Aug;7(8):1197-211.
CUB domain-containing protein 1 is a novel regulator of anoikis resistance in - CUB domain-containing protein 1 is a novel regulator of anoikis resistance in
Uekita T, Jia L, Narisawa-Saito M, Yokota J, Kiyono T, Sakai R. Mol Cell Biol. 2007 Nov;27(21):7649-60. Epub 2007 Sep 4.
Epigenetic regulation of the expression of the novel stem cell marker CDCP1 in - Epigenetic regulation of the expression of the novel stem cell marker CDCP1 in
Ikeda JI, Morii E, Kimura H, Tomita Y, Takakuwa T, Hasegawa JI, Kim YK, Miyoshi Y, Noguchi S, Nishida T, Aozasa K. J Pathol. 2006 Sep;210(1):75-84.