Anti-Cdk2 (phospho T39) antibody
| Name | Anti-Cdk2 (phospho T39) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab79379 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human, Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Hamster, Bovine, Zebrafish, Fish |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Cdk2 |
| Description | Rabbit Polyclonal |
| Gene | CDK2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Cdk2 (phospho T39) antibody (ab79379) at 1 µg/mlLane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : A431 (Human epithelial carcinoma cell lines) EGF Stimulated Whole Cell LysateLane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 6 : A431 (Human epithelial carcinoma cell lines) EGF Stimulated Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 7 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysat with Immunising peptide at 1 µg/mlLane 8 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 9 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/mlLane 10 : A431 (Human epithelial carcinoma cell lines
ICC/IF image of ab79379 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab79379 at 10µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol (100%, 5 minutes) fixed HepG2 cells at 10ug/ml