CDK2

Gene Symbol CDK2
Entrez Gene 1017
Alt Symbol CDKN2, p33(CDK2)
Species Human
Gene Type protein-coding
Description cyclin-dependent kinase 2
Other Description cdc2-related protein kinase|cell division protein kinase 2|p33 protein kinase
Swissprots O75100 P24941 A8K7C6
Accessions AAM34794 EAW96856 EAW96857 EAW96858 EAW96859 EAW96860 P24941 AA789250 AA810989 AB012305 BAA32794 AK291941 BAF84630 AK293246 BAG56780 AM393136 CAL38014 BC003065 AAH03065 BJ991087 BT006821 AAP35467 DA814453 DQ890598 ABM92215 DQ893767 ABM84693 M68520 AAA35667 X61622 CAA43807 X62071 CAA43985 XM_011537732 XP_011536034 NM_001290230 NP_001277159 NM_001798 NP_001789 NM_052827 NP_439892
Function Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1
Subcellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus, Cajal body. Cytoplasm. Endosome. Note=Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3).
Top Pathways Oocyte meiosis, Cell cycle, Measles, Herpes simplex infection, Viral carcinogenesis

CDK2 (78B2) Rabbit mAb (PE Conjugate) - 14174 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 78B2
Flow cytometric analysis of Jurkat cells using CDK2 (78B2) Rabbit mAb (PE Conjugate) and DRAQ5 ® #4084, which measures DNA content.

Phospho-CDK2 (Thr160) Antibody - 2561 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells synchronized in G0, G1/S or Mitosis, using Phospho-CDK2 (Thr160) Antibody.

CDK2 (78B2) Rabbit mAb - 2546 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 78B2
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using CDK2 (78B2) Rabbit mAb.

Cdk2 (D-12) - sc-6248 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk2 (D-12): sc-6248. Western blot analysis of Cdk2 expression in K-562 (A), A-431 (B) and NAMALWA (C) whole cell lysates.

Cdk2 (H-298) - sc-748 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cdk2 (H-298): sc-748. Western blot analysis of Cdk2 expression in NAMALWA whole cell lysate.

Cdk2 (M2) - sc-163 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cdk2 (M2): sc-163. Immunofluorescence staining of methanol-fixed SK-BR-3 cells showing nuclear localization.

Cdk2 (AN4.3) - sc-53220 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk2 (AN4.3): sc-53220. Western blot analysis of Cdk2 expression in K-562 (A), HeLa (B) and NAMALWA (C) whole cell lysates.

Cdk2 (0.N.198) - sc-70829 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk2 (0.N.198): sc-70829. Western blot analysis of Cdk2 expression in non-transfected: sc-117752 (A) and mouse Cdk2 transfected: sc-119146 (B) 293T whole cell lysates.

Cdk2 (55) - sc-136191 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Cdk2 (55): sc-136191. Western blot analysis of Cdk2 expression in non-transfected: sc-117752 (A) and human Cdk2 transfected: sc-128295 (B) 293T whole cell lysates.

p-Cdk2 (Thr 160) - sc-101656 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Cdk2 (Thr 160): sc-101656. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.

p-Cdk2/3 (Thr 160) - sc-12914 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of Cdk2/3 phosphorylation in untreated (A), nocodazole treated (B) and nocodazole and lamba protein phosphatase treated (C) HeLa whole cell lysates. Antibody tested includes p-Cdk2/3 (Thr 160)-R: sc-12914-R (A,B,C).

Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - ab183550 from Abcam

Prices $347.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17499
All lanes : Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] (ab183550) at 1/1000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 2mg/ml hydroxyurea for 24 hours.Lane 2 : Untreated HeLa whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Anti-Cdk1 + Cdk2 antibody [AN21.2] - ab6434 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone AN21.2

Anti-Cdk2 (phospho T14) antibody - ab193228 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cdk2 (phospho T14) antibody (ab193228) at 1/500 dilutionLane 1 : Lysate of HepG2 cells treated with forskolinLane 2 : Lysate of HepG2 cells treated with forskolin with antigen-specific peptide

Anti-Cdk2 (phospho T14) antibody [EP2234Y] - ab68265 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2234Y
All lanes : Anti-Cdk2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/20000 dilutionLane 1 : HeLa cell lysate, untreated.Lane 2 : HeLa cell lysate, treated with hydroxyurea.Lysates/proteins at 10 µg per lane.SecondaryHRP conjugated Goat anti-rabbit at 1/2000 dilution

Anti-Cdk2 (phospho T14) antibody - N-terminal - ab195993 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cdk2 (phospho T14) antibody - N-terminal (ab195993) at 1/500 dilutionLane 1 : Forskolin treated HepG2 whole cell lysate with antigen-specific peptideLane 2 : Forskolin treated HepG2 whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-Cdk2 (phospho T160) antibody - ab194868 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cdk2 (phospho T160) antibody (ab194868) at 1/500 dilutionLane 1 : Untreated HeLa cell extractLane 2 : UV treated HeLa cell extract

Anti-Cdk2 (phospho T160) antibody - ab195996 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cdk2 (phospho T160) antibody (ab195996) at 1/500 dilutionLane 1 : A2780 whole cell lysates with antigen-specific peptideLane 2 : A2780 whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-Cdk2 (phospho T39) antibody - ab79379 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cdk2 (phospho T39) antibody (ab79379) at 1 µg/mlLane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : A431 (Human epithelial carcinoma cell lines) EGF Stimulated Whole Cell LysateLane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 6 : A431 (Human epithelial carcinoma cell lines) EGF Stimulated Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 7 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysat with Immunising peptide at 1 µg/mlLane 8 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 9 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/mlLane 10 : A431 (Human epithelial carcinoma cell lines

Anti-Cdk2 (phospho Y15) antibody [EPR2233Y] - ab76146 from Abcam

Prices $400.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2233Y
All lanes : Anti-Cdk2 (phospho Y15) antibody [EPR2233Y] (ab76146) at 1/10000 dilutionLane 1 : Untreated HeLa cell lysateLane 2 : HeLa cells treated with alkaline phosphatase lysateLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1000

Anti-Cdk2 (phospho Y15) antibody [EPR2233Y] (Alexa Fluor® 488) - ab190193 from Abcam

Prices $437.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2233Y
ab190193 staining Cdk2 (phospho Y15) in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190193 at a working dilution of 1 in 100 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at a dilution of 1 in 250 overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Cdk2 (phospho Y15) antibody [EPR2233Y] (Alexa Fluor® 647) - ab190562 from Abcam

Prices $437.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2233Y
ab190562 staining Cdk2 (phospho Y15) in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190562 at a working dilution of 1 in 100 (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at a dilution of 1 in 250 overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Cdk2 antibody - ab6538 from Abcam

Host Rabbit
Clonality Polyclonal
SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated with ab6538 (1/200). The antibody clearly stains the nucleus (red). The cells were counterstained with DAPI (blue). 100x magnification.

Anti-Cdk2 antibody - ab7954 from Abcam

Prices $391.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal

Anti-Cdk2 antibody - ab64669 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cdk2 antibody (ab64669) at 1 µg/mlLane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : Thymus (Rat) Tissue LysateLane 4 : HeLa Whole Cell Lysate - Bleomycin Treated (20U/ml)Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 6 : Y79 (Human retinoblastoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
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