Anti-Cdk2 antibody
| Name | Anti-Cdk2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab7954 |
| Prices | $391.00 |
| Sizes | 1 ml |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IP WB ICC/IF |
| Species Reactivities | Mouse, Rat, Chicken, Human |
| Antigen | Synthetic peptide (Human) (C terminal) |
| Blocking Peptide | Cdk2 peptide |
| Description | Rabbit Polyclonal |
| Gene | CDK2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Synaptonemal complexes (red) from human spermatocytes at the pachytene stage showing CDK2 foci (green) and the kinetochore region (blue).This picture was kindly supplied as part of the review submitted by Maria Oliver-Bonet.
All lanes : Anti-Cdk2 antibody (ab7954) at 1/200 dilutionLane 1 : Whole cell lysate prepared from rat primary cortical neurons (untreated)Lane 2 : Whole cell lysate prepared from rat primary cortical neurons (treated with drug)Lysates/proteins at 20 µg per lane.SecondaryHRP conjugated goat polyclonal to rabbit Ig at 1/10000 dilutionPerformed under non-reducing conditions.
![Cdk2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Cdk2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7954.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 34kDa: Cdk2](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/26703_Cdk2-Primary-antibodies-ab7954-1.jpg)
Cdk2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Cdk2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7954.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 34kDa: Cdk2
Product References
Cyclin E is recruited to the nuclear matrix during differentiation, but is not - Cyclin E is recruited to the nuclear matrix during differentiation, but is not
Munkley J, Copeland NA, Moignard V, Knight JR, Greaves E, Ramsbottom SA, Pownall ME, Southgate J, Ainscough JF, Coverley D. Nucleic Acids Res. 2011 Apr;39(7):2671-7.
Cdk2-dependent phosphorylation of p21 regulates the role of Cdk2 in cisplatin - Cdk2-dependent phosphorylation of p21 regulates the role of Cdk2 in cisplatin
Hodeify R, Tarcsafalvi A, Megyesi J, Safirstein RL, Price PM. Am J Physiol Renal Physiol. 2011 May;300(5):F1171-9. doi:
Wee1 controls genomic stability during replication by regulating the Mus81-Eme1 - Wee1 controls genomic stability during replication by regulating the Mus81-Eme1
Dominguez-Kelly R, Martin Y, Koundrioukoff S, Tanenbaum ME, Smits VA, Medema RH, Debatisse M, Freire R. J Cell Biol. 2011 Aug 22;194(4):567-79.
Ciz1 cooperates with cyclin-A-CDK2 to activate mammalian DNA replication in - Ciz1 cooperates with cyclin-A-CDK2 to activate mammalian DNA replication in
Copeland NA, Sercombe HE, Ainscough JF, Coverley D. J Cell Sci. 2010 Apr 1;123(Pt 7):1108-15.
Protection of cisplatin cytotoxicity by an inactive cyclin-dependent kinase. - Protection of cisplatin cytotoxicity by an inactive cyclin-dependent kinase.
Hodeify R, Megyesi J, Tarcsafalvi A, Safirstein RL, Price PM. Am J Physiol Renal Physiol. 2010 Jul;299(1):F112-20. doi:
F-box protein FBXO31 mediates cyclin D1 degradation to induce G1 arrest after DNA - F-box protein FBXO31 mediates cyclin D1 degradation to induce G1 arrest after DNA
Santra MK, Wajapeyee N, Green MR. Nature. 2009 Jun 4;459(7247):722-5.
Characterization of nuclear localization signal in the N terminus of CUL4B and - Characterization of nuclear localization signal in the N terminus of CUL4B and
Zou Y, Mi J, Cui J, Lu D, Zhang X, Guo C, Gao G, Liu Q, Chen B, Shao C, Gong Y. J Biol Chem. 2009 Nov 27;284(48):33320-32.
A functional link between Wnt signaling and SKP2-independent p27 turnover in - A functional link between Wnt signaling and SKP2-independent p27 turnover in
Miranda-Carboni GA, Krum SA, Yee K, Nava M, Deng QE, Pervin S, Collado-Hidalgo A, Galic Z, Zack JA, Nakayama K, Nakayama KI, Lane TF. Genes Dev. 2008 Nov 15;22(22):3121-34.
Phosphorylation regulates SIRT1 function. - Phosphorylation regulates SIRT1 function.
Sasaki T, Maier B, Koclega KD, Chruszcz M, Gluba W, Stukenberg PT, Minor W, Scrable H. PLoS One. 2008;3(12):e4020.
An essential role for Cdk1 in S phase control is revealed via chemical genetics - An essential role for Cdk1 in S phase control is revealed via chemical genetics
Hochegger H, Dejsuphong D, Sonoda E, Saberi A, Rajendra E, Kirk J, Hunt T, Takeda S. J Cell Biol. 2007 Jul 16;178(2):257-68.