Anti-Cdk8 antibody
| Name | Anti-Cdk8 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab54561 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Recombinant fragment: QQQGNNHTNG TGHPGNQDSS HTQGPPLKKV RVVPPTTTSG GLIMTSDYQR SNPHAAYPNP GPSTSQPQSS MGYSATSQQP PQYSHQTHRY , corresponding to amino acids 375-464 of Human Cdk8 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | CDK8 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab54561 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54561, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/26912_ab54561-3-ab54561FC.jpg)
Overlay histogram showing HeLa cells stained with ab54561 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54561, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Cdk8 antibody (ab54561) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Anti-Cdk8 antibody (ab54561) at 1 µg/ml + Cell lysate prepared from mouse NIH/3T3 cells.