CDK8

Gene Symbol CDK8
Entrez Gene 1024
Alt Symbol K35
Species Human
Gene Type protein-coding
Description cyclin-dependent kinase 8
Other Description CDK8 protein kinase|cell division protein kinase 8|mediator complex subunit CDK8|mediator of RNA polymerase II transcription subunit CDK8|protein kinase K35
Swissprots Q6ISB5 Q5VUF3 P49336
Accessions EAX08384 EAX08385 EAX08386 EAX08387 P49336 AK296084 BAG58839 AK296875 BAG59437 AY702976 AAU29339 BC069634 AAH69634 BC094784 BC104492 AAI04493 BC105950 AAI05951 BC107601 AAI07602 HG518164 CDI06887 L23208 L23311 X85753 CAA59754 XM_005266215 XP_005266272 XM_005266216 XP_005266273 XM_011534863 XP_011533165 XM_011534864 XP_011533166 XM_011534865 XP_011533167 NM_001260 NP_001251
Function Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation. {ECO:0000269|PubMed:10993082, ECO:0000269|PubMed:15546612}.
Subcellular Location Nucleus {ECO:0000305}.

CDK8 (P455) Antibody - 4106 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using CDK8 (P455) Antibody.

CDK8 (G398) Antibody - 4101 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using CDK8 (G398) Antibody.

Cdk8 (C-19) - sc-1521 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Cdk8 (C-19): sc-1521. Western blot analysis of Cdk8 expression in K-562 (A), Jurkat (B), HeLa (C), NIH/3T3 (D) and KNRK (E) nuclear extracts.

Cdk8 (D-9) - sc-13155 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk8 (D-9): sc-13155. Western blot analysis of Cdk8 expression in K-562 nuclear extract.

Cdk8 (H-139) - sc-5612 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cdk8 (H-139): sc-5612. Western blot analysis of Cdk8 expression in K-562 nuclear extract.

Cdk8 (S-10) - sc-32493 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Cdk8 (D-12) - sc-32492 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Cdk8 antibody - ab54561 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HeLa cells stained with ab54561 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54561, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Cdk8 antibody - ab106320 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cdk8 antibody (ab106320) at 1 µg/ml (Milk Blocked - 5%)Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 6 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell LysateLane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Cdk8 antibody - ab115155 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunoprecipitation of Cdk8 (3 µg of antibody per mg of HeLa cells lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with anti-Cdk8 antibody (ab115155); Lane 2-Immunoprecipitation with an anti-Cdk8 antibody that recognizes an upstream epitope; Lane 3- Immunoprecipitation with IgG control. An anti-Cdk8 antibody used in lane 2 was used at 0.4 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 30 sec.

Anti-Cdk8 antibody - ab123940 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Cdk8 antibody (ab123940) at 1/100 dilution + K562 cell line lysate at 35 µg

Anti-Cdk8 antibody - ab64940 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cdk8 antibody (ab64940) at 1/500 dilutionLane 1 : extracts from 293 cells (5-30µg total protein)Lane 2 : extracts from 293 cells (5-30µg total protein) and 5-10µg of the immunising peptide.

Anti-Cdk8 antibody - ab124218 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab124218 at 1/250 dilution, staining Cdk8 in Formalin-fixed Paraffin-embedded Human Breast carcinoma by Immunohistochemistry. Detection: DAB staining.

Anti-Cdk8 antibody [EPR3699(N)] - N-terminal - ab176559 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3699(N)
All lanes : Anti-Cdk8 antibody [EPR3699(N)] - N-terminal (ab176559) at 1/10000 dilutionLane 1 : Caco 2 cell lysateLane 2 : K562 cell lysateLane 3 : HeLa cell lysateLane 4 : HCT-116 cell lysateLysates/proteins at 10 µg per lane.developed using the ECL technique

Anti-CDK8 Antibody - LS-C6017 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-CDK8 Antibody (aa375-464) - LS-C190667 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-CDK8 Antibody (C-Terminus) - LS-C31103 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
HepG2 Cell Lysate

Anti-CDK8 Antibody (aa244-272, APC) - LS-C254466 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CDK8 Antibody (aa244-272, AP) - LS-C254465 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CDK8 Antibody (aa244-272, Biotin) - LS-C254470 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CDK8 Antibody (aa244-272, FITC) - LS-C254471 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CDK8 Antibody (aa1-50) - LS-C118905 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from 293 cells, using CDK8 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-CDK8 Antibody (aa244-272, PE) - LS-C254472 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CDK8 Antibody (aa244-272, Azide-free, HRP) - LS-C254473 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CDK8 Antibody (aa3-32, AP) - LS-C254474 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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