Standard Curve for Cdk9 (Analyte: Cdk9 protein (Tagged) (ab85603)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to Cdk9 (ab76873) at 5µg/ml and Detector Antibody Rabbit polyclonal to Cdk9 (ab38840) at 0.5µg/ml.
Anti-Cdk9 antibody (ab76873) at 1 µg/ml + immunogen (GST-tagged 102aa recombinant fragment) at 0.1 µgSecondaryGoat Anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution
All lanes : Anti-Cdk9 antibody (ab76873) at 1 µg/mlLane 1 : Cdk9-transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution