CDK9

Gene Symbol CDK9
Entrez Gene 1025
Alt Symbol C-2k, CDC2L4, CTK1, PITALRE, TAK
Species Human
Gene Type protein-coding
Description cyclin-dependent kinase 9
Other Description CDC2-related kinase|cell division cycle 2-like protein kinase 4|cell division protein kinase 9|serine/threonine protein kinase PITALRE|tat-associated kinase complex catalytic subunit
Swissprots Q96TF1 P50750 Q5JU24 Q5U006 Q5JU25
Accessions AAF72183 AAM54039 EAW87696 EAW87697 P50750 AB451289 BAG70103 AB451421 BAG70235 AK313828 BAG36563 BC001968 AAH01968 BE549667 BT019903 AAV38706 BX091973 DB120461 DQ891182 ABM82108 DQ894364 ABM85290 DQ894365 ABM85291 L25676 AAA35668 X80230 CAA56516 NM_001261 NP_001252
Function Protein kinase involved in the regulation of transcription. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR, and the negative elongation factors DSIF and NELF. Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis. P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export. Modulates a complex network of chromatin modificatio
Subcellular Location Nucleus. Cytoplasm. Nucleus, PML body. Note=Accumulates on chromatin in response to replication stress. Complexed with CCNT1 in nuclear speckles, but uncomplexed form in the cytoplasm. The translocation from nucleus to cytoplasm is XPO1/CRM1-dependent. Associates with PML body when acetylated.
Tissue Specificity Ubiquitous.
Top Pathways Transcriptional misregulation in cancer

Phospho-CDK9 (Thr186) Antibody - 2549 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types, untreated or treated with λ phosphatase and calf intestinal phosphatase (CIP), using Phospho-CDK9 (Thr186) Antibody (upper) or CDK9 (C12F7) Rabbit mAb #2316 (lower).

CDK9 (C12F7) Rabbit mAb - 2316 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C12F7
Western blot analysis of extracts from various cell types using CDK9 (C12F7) Rabbit mAb.

Cdk9 (C-20) - sc-484 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cdk9 (C-20): sc-484. Western blot analysis of Cdk9 expression in HL-60 whole cell lysate.

Cdk9 (H-169) - sc-8338 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cdk9 (H-169)): sc-8338. Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

Cdk9 (D-7) - sc-13130 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk9 (D-7): sc-13130. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lymphoma showing nuclear staining.

Cdk9 (F-6) - sc-376646 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk9 (F-6): sc-376646. Western blot analysis of Cdk9 expression in HL-60 (A) and NIH/3T3 (B) whole cell lysates.

Cdk9 (H-1) - sc-393422 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk9 (H-1): sc-393422. Western blot analysis of Cdk9 expression in HL-60 (A), NIH/3T3 (B) and HeLa (C) whole cell lysates and KNRK (D) and HeLa (E) nuclear extracts.

Cdk9 (E-4) - sc-376624 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk9 (E-4): sc-376624. Western blot analysis of Cdk9 expression in HL-60 whole cell lysate.

Cdk9 (L-19) - sc-7331 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Cdk9 (L-19): sc-7331. Western blot analysis of Cdk9 expression in non-transfected: sc-110760 (A) and human Cdk9 transfected: sc-110469 (B) 293 whole cell lysates.

Anti-Cdk9 (phospho T186) antibody - ab79178 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cdk9 (phospho T186) antibody (ab79178)Lane 1 : 3T3 cells treated with Forskolin (40 nM, 30 minutes) Lane 2 : 3T3 cells treated with Forskolin (40 nM, 30 minutes) with phospho peptide

Anti-Cdk9 antibody - ab6544 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum. The staining shows the location of cdk9 / PITALRE protein in developing mouse tissue. Arrow indicates area of high expression.

Anti-Cdk9 antibody - ab38840 from Abcam

Prices $384.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Standard Curve for Cdk9 (Analyte: Cdk9 protein (Tagged) (ab85603)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to Cdk9 (ab76873) at 5µg/ml and Detector Antibody Rabbit polyclonal to Cdk9 (ab38840) at 0.5µg/ml.

Anti-Cdk9 antibody - ab10874 from Abcam

Prices $372.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-Cdk9 antibody - ab39098 from Abcam

Prices $376.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cdk9 antibody (ab39098) at 1/250 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Cdk9 antibody - ab76873 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Standard Curve for Cdk9 (Analyte: Cdk9 protein (Tagged) (ab85603)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to Cdk9 (ab76873) at 5µg/ml and Detector Antibody Rabbit polyclonal to Cdk9 (ab38840) at 0.5µg/ml.

Anti-Cdk9 antibody - ab124261 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Cdk9 antibody which recognizes a downstream epitope of Cdk9 was used at 1 µg/ml detecting Cdk9 in HeLa whole cell lysate by WB following IP. Lane 1: IP with ab124261 at 6 µg/mg of lysate.Lane 2: IP with an antibody which recognizes an downstream epitope of Cdk9.Lane 3: Control IgG. In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded. Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-Cdk9 antibody [1B5A7] - ab113666 from Abcam

Host Mouse
Clonality Monoclonal
Clone 1B5A7
All lanes : Anti-Cdk9 antibody [1B5A7] (ab113666) at 1/500 dilutionLane 1 : HEK293 cell lysate, non transfected Lane 2 : HEK293 cell lysate, transfected with Human Cdk9-hIgGFc

Anti-Cdk9 antibody [1B5A7] - ab130230 from Abcam

Host Mouse
Clonality Monoclonal
Clone 1B5A7
All lanes : Anti-Cdk9 antibody [1B5A7] (ab130230) at 1/500 dilutionLane 1 : HEK293 cell lysateLane 2 : Cdk9-hIgGFc transfected HEK293 cell lysate.

Anti-Cdk9 antibody [EP3118] - ab75848 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP3118
ab75848 at 1/100 dilution staining Cdk9 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue.

Anti-Cdk9 antibody [EPR3119Y] - ab76320 from Abcam

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3119Y
All lanes : Anti-Cdk9 antibody [EPR3119Y] (ab76320) at 1/2000 dilutionLane 1 : HT-29 cell lysateLane 2 : HL-60 cell lysateLane 3 : HeLa cell lysateLane 4 : 293 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/1000 dilution

Anti-CDK9 Antibody - LS-C6020 from LifeSpan Bioscience

Prices $415.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-CDK9 Antibody (C-Terminus) - LS-C79012 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal

Anti-CDK9 Antibody (phospho-Thr186) - LS-C79013 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CDK9 Antibody (C-Terminus) - LS-C125338 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-CDK9 Antibody (N-Terminus) - LS-C31810 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Transfected 293T Cell Lysate
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