Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse hybridoma tumor (right) tissues labelling CDT2 with ab72264 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-CDT2 antibody (ab72264) at 0.04 µg/mlLane 1 : Whole cell lysate from Hela cells at 50 µgLane 2 : Whole cell lysate from Hela cells at 15 µgLane 3 : Whole cell lysate from Hela cells at 5 µgLane 4 : Whole cell lysate from 293T cells at 50 µg
Immunoprecipitation/ Western Blot of CDT2. Lane 1: ab72264 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab72264 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 10 seconds.
ICC/IF image of ab72264 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72264, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.