DTL

Gene Symbol DTL
Entrez Gene 51514
Alt Symbol CDT2, DCAF2, L2DTL, RAMP
Species Human
Gene Type protein-coding
Description denticleless E3 ubiquitin protein ligase homolog (Drosophila)
Other Description DDB1 and CUL4 associated factor 2|DDB1- and CUL4-associated factor 2|RA regulated nuclear matrix associated protein|RA-regulated nuclear matrix-associated protein|denticleless protein homolog|lethal(2) denticleless protein homolog|retinoic acid-regulated nuclear matrix-associated protein
Swissprots Q9NWM5 Q9NW34 Q9NW03 Q96SN0 Q9NZJ0 D3DT98 A8K8H8 Q5VT77
Accessions EAW93395 EAW93396 EAW93397 EAW93398 Q9NZJ0 AF195765 AAF35182 AF345896 AAK54706 AI800751 AK000742 BAA91355 AK001206 BAA91552 AK001261 BAA91586 AK027651 BAB55267 AK292343 BAF85032 AK299649 BAG61569 AK303346 BAG64408 BC033297 AAH33297 BC033540 AAH33540 BF508267 DB070033 DQ641253 ABG23317 JF432257 ADZ15474 XM_011509614 XP_011507916 NM_001286229 NP_001273158 NM_001286230 NP_001273159 NM_016448 NP_057532
Function Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1) and SETD8. CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. SETD8 degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(
Subcellular Location Nucleus. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome. Note=Nuclear matrix-associated protein. Translocates from the interphase nucleus to the metaphase cytoplasm during mitosis.
Tissue Specificity Expressed in placenta and testis, very low expression seen in skeletal muscle. Detected in all hematopoietic tissues examined, with highest expression in thymus and bone marrow. A low level detected in the spleen and lymph node, and barely detectable level in the peripheral leukocytes. RA treatment down-regulated the expression in NT2 cell. {ECO:0000269|PubMed:11278750, ECO:0000269|PubMed:17106265}.

Cdt2 (B-8) - sc-166735 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdt2 (B-8): sc-166735. Western blot analysis of Cdt2 expression in non-transfected: sc-117752 (A) and human Cdt2 transfected: sc-113907 (B) 293T whole cell lysates.

Cdt2 (E-20) - sc-79901 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Cdt2 (H-240) - sc-98442 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Cdt2 (N-14) - sc-79903 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-CDT2 antibody - ab72264 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse hybridoma tumor (right) tissues labelling CDT2 with ab72264 at 1/1000 (1µg/ml). Detection: DAB.

Anti-CDT2 antibody - ab174385 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labelling CDT2 with ab174385  at 1/100 dilution (Red). The cells were stained for DNA with Hoechst 33258 (Blue). CDT2 protein is localized in nuclei at all cell stages.

Anti-CDT2 antibody - ab95129 from Abcam

Prices $380.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab95129, at 1/250 dilution, staining CDT2 in formalin-fixed, paraffin-embedded Human colon carcinoma by Immunohistochemistry, using DAB staining.

Anti-CDT2 antibody - ab199292 from Abcam

Prices $376.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CDT2 antibody (ab199292) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : MCF7 cell lysateLane 3 : U373 cell lysate

Anti-CDT2 antibody [EPR14978] - ab184548 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14978
Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling CDT2 using ab184548 at a 1/240 dilution (red) with negative control, Rabbit monoclonal IgG (green). Secondary antibody, Goat anti rabbit IgG (FITC) at a 1/150 dilution.

Anti-DTL / CDT2 Antibody (C-Terminus) - LS-C65048 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-DTL / CDT2 Antibody - LS-C96092 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Chicken
Clonality Polyclonal

Anti-DTL / CDT2 Antibody (aa229-256, APC) - LS-C234769 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DTL / CDT2 Antibody (aa229-256, Biotin) - LS-C234770 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DTL / CDT2 Antibody (aa229-256, FITC) - LS-C234771 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DTL / CDT2 Antibody (aa229-256, PE) - LS-C234775 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DTL / CDT2 Antibody (aa229-256, AP) - LS-C234772 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DTL / CDT2 Antibody (aa229-256, Azide-free, HRP) - LS-C249871 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DTL / CDT2 Antibody (aa182-231) - LS-C80868 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-DTL / CDT2 Antibody - LS-C310175 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DTL / CDT2 Antibody (aa1-196) - LS-C311890 from LifeSpan Bioscience

Prices $250.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DTL / CDT2 Antibody (aa229-256) - LS-C320961 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DTL / CDT2 Antibody (aa375-425) - LS-C287779 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human DTL/CDT2 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-DTL/CDT2 used at a dilution of 1:1000 (1 Detection: DAB.

Anti-DTL / CDT2 Antibody (aa229-256) - LS-C167549 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
DTL Antibody western blot of mouse spleen tissue lysates (35 ug/lane). The DTL antibody detected the DTL protein (arrow).

Anti-DTL / CDT2 Antibody (C-Terminus) - LS-C287781 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse DTL/CDT2 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma (left) and mouse hybridoma tumor (right). Antibody: Affinity purified rabbit anti-DTL/CDT2 used at a dilution of 1:1000 (1 Detection: DAB.

Anti-DTL / CDT2 Antibody (C-Terminus) - LS-C286712 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse DTL/CDT2 by Immunohistochemistry. Sample: FFPE section of mouse hybridoma tumor. Antibody: Affinity purified rabbit anti-DTL/CDT2 used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.
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