Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling CDT2 using ab184548 at a 1/240 dilution (red) with negative control, Rabbit monoclonal IgG (green). Secondary antibody, Goat anti rabbit IgG (FITC) at a 1/150 dilution.
Immunofluorescent staining of 4% paraformaldehyde fixed HeLa cells labeling CDT2 using ab184548 at a 1/100 dilutiion and Goat anti rabbit IgG (Alexa Fluor® 555) secondary at a 1/200 dilution (red). Counterstained with Dapi (blue).
Immunohistochemical analysis of paraffin-embedded sections of Human testis tissue labeling CDT2 using ab148548 at a1/100 dilution, counterstained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded sections of Human thymus tissue labeling CDT2 using ab148548 at a1/100 dilution, counterstained with Hematoxylin.
Anti-CDT2 antibody [EPR14978] (ab184548) at 1/10000 dilution + HeLa cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-CDT2 antibody [EPR14978] (ab184548) at 1/50000 dilutionLane 1 : 293 cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution