Cdk4 Antibody (C-22): sc-260-G. Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic and nuclear staining of glandular cells and interstitial cells.
Cdk4 (C-22)-G: sc-260-G. Western blot analysis of Cdk4 expression in non-transfected 293T: sc-117752 (A), mouse Cdk4 transfected 293T: sc-119150 (B) and HeLa (C) whole cell lysates.
MHC (G-4): sc-6956. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoskeletal rhodamine immunostaining of myosin heavy chain. Note also nuclear rhodamine immunostaining with Cdk4 (C-22): sc-260.
Cdk4 (C-22)-G: sc-260-G. Western blot analysis of Cdk4 expression in Ramos whole cell lysate.
Cdk4 (C-22): sc-260. Western blot analysis of Cdk4 expression in non-transfected 293T: sc-117752 (A), mouse Cdk4 transfected 293T: sc-119150 (B) and HeLa (C) whole cell lysates.
Cdk4 (C-22): sc-260. Western blot analysis of Cdk4 p34 expression in HeLa whole cell lysate.
Cdk4 (C-22): sc-260. Western blot analysis of Cdk4 expression in NIH/3T3 nuclear extract.
Cdk4 siRNA (h): sc-29261. Western blot analysis of Cdk4 expression in non-transfected control (A) and Cdk4 siRNA transfected (B) HeLa cells. Blot probed with Cdk4 (C-22): sc-260. Actin (I-19): sc-1616 used as specificity and loading control.
Immunoprecipitation of Cdk4 from HeLa whole cell lysate using Cdk4 (DCS-35): sc-23896 (mouse monoclonal antibody) followed by Western blot analysis using Cdk4 (C-22)-G: sc-260-G (goat polyclonal antibody). Note presence of Protein A/G band using Protein A/G PLUS-Agarose: sc-2003 conventional IP matrix (A) as compared to their absence using ExactaCruz A: sc-45038 (B).
Cdk4 (C-22): sc-260. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human skin. Note nuclear staining of epidermal cells.
Cdk4 (C-22): sc-260. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear localization.