CDK4

Gene Symbol CDK4
Entrez Gene 1019
Alt Symbol CMM3, PSK-J3
Species Human
Gene Type protein-coding
Description cyclin-dependent kinase 4
Other Description cell division protein kinase 4
Swissprots Q6FG61 P11802 B4DNF9 O00576 B2R9A0
Accessions AAC39521 AAC50506 AAD13991 AAM23014 CDI30203 CDM63717 EAW97058 EAW97059 EAW97060 EAW97061 EAW97062 P11802 AK297901 BAG60221 AK310724 AK313701 BAG36447 BC003644 AAH03644 BC005864 AAH05864 BC010153 AAH10153 BC015669 AAH15669 BI832658 BQ773564 CR407668 CAG28596 CR542247 CAG47043 DQ893137 ABM84063 DQ896420 ABM87419 M14505 AAA35673 U79269 AAB50213 Z48970 CAA88834 NM_000075 NP_000066 NM_052984
Function Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. {ECO:0000269|PubMed:15241418, ECO:0000269|PubMed:18827403, ECO:0000269|PubMed:9003781}.
Subcellular Location Cytoplasm. Nucleus. Membrane. Note=Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
Top Pathways p53 signaling pathway, Tight junction, PI3K-Akt signaling pathway, T cell receptor signaling pathway, Viral carcinogenesis

CDK4 (D9G3E) Rabbit mAb - 12790 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9G3E
Western blot analysis of extracts from various cell lines using CDK4 (D9G3E) Rabbit mAb.

CDK4 (DCS156) Mouse mAb - 2906 from Cell Signaling Technology

Host Mouse
Clonality Monoclonal
Clone DCS156
Western blot analysis of extracts from SK-N-MC and IMCD3 cells, using CDK4 (DCS156) Mouse mAb.

Phospho-CDK4 (Thr172) Antibody - 5884 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HT-29 cells, untreated or synchronized in G1 phase, using Phospho-CDK4 (Thr172) Antibody (upper) or CDK4 (DCS156) Mouse mAb #2906 (lower). After western transfer, the membrane was either left untreated (-CIP, left) or treated with Calf Intestinal Phosphatase (+CIP, right). G1 phase synchronization was performed by thymidine block (2 mM, 16 hours), release into nocodazole (100 ng/mL, 24 hours), selective detachment of mitotic cells, and release into G1 phase (4 hours).

Cdk4 (C-22) - sc-260 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cdk4 Antibody (C-22): sc-260-G. Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic and nuclear staining of glandular cells and interstitial cells.

Cdk4 (DCS-35) - sc-23896 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk4 (DCS-35): sc-23896. Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

Cdk4 (F-8) - sc-393653 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk4 (F-8): sc-393653. Western blot analysis of Cdk4 expression in non-transfected 293T: sc-117752 (A), mouse Cdk4 transfected 293T: sc-119150 (B), SK-BR-3 (C), HeLa (D) and Ramos (E) whole cell lysates.

Cdk4 (H-22) - sc-601 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cdk4 (H-22)-G: sc-601-G. Western blot analysis of Cdk4 expression in HeLa (A), Ramos (B) and K-562 (C) whole cell lysates.

Cdk4 (B-10) - sc-166373 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk4 (B-10): sc-166373. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic and nuclear localization.

Cdk4 (H-303) - sc-749 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cdk4 (H-303): sc-749. Western blot analysis of Cdk4 expression in HeLa whole cell lysate.

Cdk4 (97) - sc-136241 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Cdk4 (97): sc-136241. Western blot analysis of Cdk4 expression in RSV-3T3 whole cell lysate.

Cdk4 (DCS-31) - sc-56277 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk4 (DCS-31): sc-56277. Western blot analysis of Cdk4 expression in MCF7 (A) and HeLa (B) whole cell lysates.

Cdk4 (DCS-156) - sc-53636 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk4 (DCS-156): sc-53636. Western blot analysis of Cdk4 expression in MCF7 whole cell lysate.

Cdk4 (3F121) - sc-70831 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk4 (3F121): sc-70831. Western blot analysis of Cdk4 expression in non-transfected 293T: sc-117752 (A), mouse Cdk4 transfected 293T: sc-119150 (B) and HeLa (C) whole cell lysates.

Cdk4 (0.N.199) - sc-70832 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk4 (0.N.199): sc-70832. Western blot analysis of Cdk4 expression in non-transfected 293T: sc-117752 (A), mouse Cdk4 transfected 293T: sc-119150 (B) and HeLa (C) whole cell lysates.

Cdk4 (SPM382) - sc-56361 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk4 (SPM382): sc-56361. Western blot analysis of Cdk4 expression in HeLa whole cell lysate.

Anti-Cdk4 antibody - ab7955 from Abcam

Prices $398.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab7955 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7955, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Cdk4 antibody - ab137675 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Cdk4 antibody (ab137675) at 1/1000 dilution + 293T whole cell lysate at 30 µg

Anti-Cdk4 antibody - ab6551 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-Cdk4 antibody - ab95255 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Cdk4 antibody (ab95255) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Cdk4 antibody - ab54515 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-Cdk4 antibody - ab54517 from Abcam

Host Mouse
Clonality Monoclonal

Anti-Cdk4 antibody [3F9-B12-C8] - ab131197 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F9-B12-C8
All lanes : Anti-Cdk4 antibody [3F9-B12-C8] (ab131197) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : LNCAP cell lysateLane 3 : F9 cell lysate

Anti-Cdk4 antibody [AF10A4] - ab75511 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone AF10A4
Standard Curve for Cdk4 (Analyte: Cdk4 protein (T7 Tag) (ab40586)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [AF10A4] to Cdk4 (ab75511) at 1/200 dilution. and Detector Antibody Rabbit polyclonal to Cdk4 (ab7955) at 0.5µg/ml.

Anti-Cdk4 antibody [DCS-31] - ab6315 from Abcam

Prices $392.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone DCS-31
ab6315 staining human MDA-MB-436 breast cancer cells by Flow Cytometery. Cells were treated with Trypsin/EDTA for 5 minutes, sample buffer prepared as 5%FCS in PBS and gating was done on live non apoptotic cells. The primary antibody diluted 1/500 and incubated with sample for 1 hour at 4°C. A FITC conjugated horse polyclonal to mouse IgG, diluted 1/200 was used as secondary

Anti-Cdk4 antibody [DCS-31] - ab77672 from Abcam

Prices $372.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone DCS-31
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