Anti-CDX2 antibody [AMT28]
| Name | Anti-CDX2 antibody [AMT28] |
|---|---|
| Supplier | Abcam |
| Catalog | ab15258 |
| Prices | $382.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | AMT28 |
| Applications | ICC/IF IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Prokaryotic recombinant protein corresponding to the 180 amino acid N-terminal region of the human CDX2 molecule |
| Description | Mouse Monoclonal |
| Gene | CDX2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab15258 staining human normal colon. Staining is localised to the nucleus.Left panel: with primary antibody at a dilution of 1:1000. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab15258 staining CDX2 in human HT29 Colorectal Cancer cells by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton x100 and blocking with 1% serum was done for 1 hour at 250C. Samples were incubated with primary antibody (1/100: in 1/100 serum in PBS-T) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated goat polyclonal to mouse IgG (H+L) horseradish peroxidase conjugate was used at dilution at 1/100 as secondary antibody.See Abreview
Product References
Changes in sub-cellular localisation of trophoblast and inner cell mass specific - Changes in sub-cellular localisation of trophoblast and inner cell mass specific
Madeja ZE, Sosnowski J, Hryniewicz K, Warzych E, Pawlak P, Rozwadowska N, Plusa B, Lechniak D. BMC Dev Biol. 2013 Aug 13;13:32.
Generation of anterior foregut endoderm from human embryonic and induced - Generation of anterior foregut endoderm from human embryonic and induced
Green MD, Chen A, Nostro MC, d'Souza SL, Schaniel C, Lemischka IR, Gouon-Evans V, Keller G, Snoeck HW. Nat Biotechnol. 2011 Mar;29(3):267-72.
Role of SOX2 in maintaining pluripotency of human embryonic stem cells. - Role of SOX2 in maintaining pluripotency of human embryonic stem cells.
Adachi K, Suemori H, Yasuda SY, Nakatsuji N, Kawase E. Genes Cells. 2010 May;15(5):455-70.
Characterization of rectal, proximal and distal colon cancers based on - Characterization of rectal, proximal and distal colon cancers based on
Minoo P, Zlobec I, Peterson M, Terracciano L, Lugli A. Int J Oncol. 2010 Sep;37(3):707-18.
Aldehyde dehydrogenase-expressing colon stem cells contribute to tumorigenesis in - Aldehyde dehydrogenase-expressing colon stem cells contribute to tumorigenesis in
Carpentino JE, Hynes MJ, Appelman HD, Zheng T, Steindler DA, Scott EW, Huang EH. Cancer Res. 2009 Oct 15;69(20):8208-15.
Ets transcription factors control epithelial maturation and transit and - Ets transcription factors control epithelial maturation and transit and
Jedlicka P, Sui X, Sussel L, Gutierrez-Hartmann A. Am J Pathol. 2009 Apr;174(4):1280-90.
Pancreatic endoderm derived from human embryonic stem cells generates - Pancreatic endoderm derived from human embryonic stem cells generates
Kroon E, Martinson LA, Kadoya K, Bang AG, Kelly OG, Eliazer S, Young H, Richardson M, Smart NG, Cunningham J, Agulnick AD, D'Amour KA, Carpenter MK, Baetge EE. Nat Biotechnol. 2008 Apr;26(4):443-52.
Efficient differentiation of human embryonic stem cells to definitive endoderm. - Efficient differentiation of human embryonic stem cells to definitive endoderm.
D'Amour KA, Agulnick AD, Eliazer S, Kelly OG, Kroon E, Baetge EE. Nat Biotechnol. 2005 Dec;23(12):1534-41. Epub 2005 Oct 28.